Generi Biotech - Compounds and Services for Molecular Biology

• Quantitative PCR
  • Sets for Gene Expression
  • qPCR sets for reference genes
  • Custom-Made qPCR Sets
  • Probe Master Mix
  • Standards for qPCR
  • Internal Positive Controls
• Sequencing
  • Standard Sequencing
  • 16S/ITS Sequencing
• Molecular Cloning
  • Custom cloning
  • Plasmid Amplification and Isolation
  • Competent Bacteria
• Cell Culture Applications
  • Recombinant Proteins
  • Recombinant Human SHBG
  • Cytotoxicity Testing
  • Luminescence Solution
  • Mycoplasma Detection
• Biological Identification
  • Cell Line Authentication
  • Paternity Outsourcing

Model Examples of Cloning

Example 1: Single-step recloning of the gene X coding sequence from plasmid pA to plasmid pB

The price includes restriction of both plasmids, DNA fragments isolation from the agarose gel, ligation, transformation, screening bacterial colonies by restriction, and midiprep.

Price: EUR 418,20 (excl. VAT)

Example 2: Single-step cloning of the insert into the vector using PCR

The price includes single-step cloning with amplification of the insert sized 1 kb using "proofreading" polymerase, including design and synthesis of primers and PCR optimization. Furthermore, the price includes design of the plasmid cloning scheme together with optimization of the requirement for "in frame" cloning in of the insert.

Price: EUR 1 258,50 (excl. VAT)

Example 3: Three-step cloning of the plasmid using a linker

The price includes three-step cloning where in one of the steps, a linker has to be designed and cloned in, with the necessary recognition sites for restriction enzymes. Furthermore, the price includes design of the plasmid cloning scheme.

Price: EUR 1 714,40 (excl. VAT)