Generi Biotech - Compounds and Services for Molecular Biology

• Quantitative PCR
  • Sets for Gene Expression
  • qPCR sets for reference genes
  • Custom-Made qPCR Sets
  • Probe Master Mix
  • Standards for qPCR
  • Internal Positive Controls
• Sequencing
  • Standard Sequencing
  • 16S/ITS Sequencing
• Molecular Cloning
  • Custom cloning
  • Plasmid Amplification and Isolation
  • Competent Bacteria
• Cell Culture Applications
  • Recombinant Proteins
  • Recombinant Human SHBG
  • Cytotoxicity Testing
  • Luminescence Solution
  • Mycoplasma Detection
• Biological Identification
  • Cell Line Authentication
  • Paternity Outsourcing

Detailed Specifications of Supplied E. coli Strains

E. coli JM109

Genotype: F´ traD36 proA⁺B⁺ lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14^- gyrA96 recA1 relA1 endA1 thi hsdR17
Recommended Growth Medium: M9
Growth Temperature: 37 °C
dam/dcm Methylation: dam+, dcm+
Protease-deficient: No
Lacl^q: Yes
Drug Resistance: nal
Suitable for cloning and subcloning, blue/white screening.

E. coli TB1

Genotype: F^- ara Δ(lac-proAB) [Φ80dlac Δ(lacZ)M15] rpsL(Str^R) thi hsdR
Recommended Growth Medium: LB
Growth Temperature: 37 °C
dam/dcm Methylation: dam+, dcm+
Protease-deficient: No
Lacl^q: No
Drug Resistance: str
Suitable for protein expression.

E. coli DH5α

Genotype: F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK^- mK⁺), λ-
Recommended Growth Medium: LB
Growth Temperature: 37 °C
Drug Resistance: nal
Suitable for cloning and subloning, blue/white screening.