|
Dry ice delivery
DetailsThe sample was kept inside standard shipping parcel with polystyrene thermoinsulating blocks until temperature increased to -26°C (after 53 hours). At time zero, 2680g of dry ice in 2 pieces (1 and 1/3 of block) were put inside a parcel, together with a control sample of competent cells whose initial competency was 2.3 * 107. After 53 hours, the dry ice evaporated to a final weight of 306 g. Competency of cells kept inside the parcel was measured again and set to 2.0 * 107. The temperature inside the parcel was measured with temperature data logger SM 325 (Dickson, Addison, IL, USA), using thermocouple probe placed into a standard commercial paper box (together with a control sample of competent cells), in which competent cells are currently sold. Parcel was left at ambient temperature 24°C (+/-1°C) without movement excluding its weighing.
Plot
DetailsThe sample was kept inside standard shipping parcel with polystyrene thermoinsulating blocks until temperature increased to -28.3°C (after 77 hours). At time zero, 3630g of dry ice in 4 pieces (1 and 3/4 of block) were put inside a parcel, together with a control sample of competent cells whose initial competency was 2.3 * 107. After 77 hours, the dry ice evaporated to a final weight of 292 g. Competency of cells kept inside the parcel was measured again and set to 1.5 * 107. The temperature inside the parcel was measured with temperature data logger SM 325 (Dickson, Addison, IL, USA), using thermocouple probe placed into a standard commercial paper box (together with a control sample of competent cells), in which competent cells are currently sold. Parcel was left at ambient temperature 24°C (+/-1°C) without movement excluding its weighing.
Plot
DetailsThe sample was kept inside standard shipping parcel with polystyrene thermoinsulating blocks until temperature increased to -22°C (after 53 hours). At time zero, a 2204g-block of dry ice was put inside a parcel, together with a control sample of competent cells whose initial competency was 2.3 * 107. After 53 hours, the dry ice evaporated to a final weight of 136 g. Competency of cells kept inside the parcel was measured again and set to 1.1 * 107. The temperature inside the parcel was measured with temperature data logger SM 325 (Dickson, Addison, IL, USA), using thermocouple probe placed into a standard commercial paper box (together with a control sample of competent cells), in which competent cells are currently sold. Parcel was left at ambient temperature 24°C (+/-1°C) without movement excluding its weighing.
Plot
|