Examples of HPLC ChromatogramsChromatograms of the oligonucleotide 5´ CAGAGCAAGACTTCATCT 3´ labelled with fluorescein at the end 5´. The upper figure shows a chromatogram upon UV light detection (260 nm); the bottom figure shows a chromatogram upon visible light range detection (480 nm). The left peak in the visible light disappears; it is a fraction of an unlabelled oligonucleotide, while the peak on the right is present both in the UV and visible light - it is a fraction of an oligonucleotide with the fluorescein molecule.
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