Generi Biotech - Compounds and Services for Molecular Biology

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Examples of MALDI-TOF Spectrograms

Analysed using the device MALDI-TOF Autoflex II (Bruker) in the linear mode, using the trap MTP AnchorChipTM and 3-hydroxypicolinic acid as the matrix.

Oligonucleotide seqB is a very high-quality and no sequential impurities are present. Oligonucleotide seqA is a very low-quality oligonucleotide where full coupling efficiency (especially of G and A bases) was not achieved and the oligonucleotide sequence can be "read".

Note: A similar record (as in seqA) can be intentionally achieved upon sequencing of oligonucleotides using the MALDI-TOF technique, so called "in-source decay".

High-quality oligo - SeqB

Low-quality oligo - SeqA