Overview of Modifications at the 3´ End

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Overview of Modifications at the 3´ End

BIOTIN (3´ end)

Application	As a labelling molecule
Modification structure	Biotin molecule is separated from the 3´ end of the oligonucleotide chain by a 15-carbon spacer
Available purification types	OPC, HPLC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 52 / oligo (VAT excluded)

BLOCKADE OF THE 3´ EXONUCLEASE ACTIVITY

Blockade of the 3´ exonuclease activity can be performed using modification of the oligonucleotide 3´ end by the PRIMARY AMINO GROUP >> or THIOL GROUP >>

BLOCKADE OF THE 3´ POLYMERASE EXTENSION (3´ end)

Application	Oligonucleotide with blockade of the 3´-hydroxyl group at the end 3´ is not capable of extension (PCR or RT-PCR)
Modification structure	3-carbon 3´-spacer is used for the blockade. Blockade of the 3´-OH group can be used for the same purpose, using 2´, 3´-dideoxynucleosides or 3´-deoxynucleosides; however, this procedure is several-fold more expensive.
Available purification types	OPC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 42 / oligo (VAT excluded)

DABCYL (3´ end)

Application	As a labelling molecule; universal quencher
Modification structure	The molecule of 4-(dimethylamino)-azobenzene is bound to the 3´ end of the oligonucleotide by a 9-carbon spacer
Available purification types	OPC
Term of delivery	X
Extra fee for labelling for 200 nmol	EUR 52 / oligo (VAT excluded)

FLUORESCEIN (3´ end)

Application	As a labelling molecule
Modification structure	The molecule of aminofluorescein is separated from the 3´ end of the oligonucleotide chain by a 6-carbon spacer
Available purification types	OPC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 52 / oligo (VAT excluded)

CHOLESTEROL (3´ end)

Application	To increase lipophilic characteristic of the oligonucleotide; in antisense oligonucleotides (phosphorothioates and/or other derivatives), to increase permeance of oligonucleotide chains through cellular membranes
Modification structure	Cholesterol molecule is separated from the 3´ end of the oligonucleotide chain by a 15-carbon spacer
Available purification types	OPC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 52 / oligo (VAT excluded)

PRIMARY AMINO GROUP (3´ end)

Application	As an intermediate stage for further adjustments of the oligonucleotide (bond to special surfaces, conjugation) while preserving the 5´ end for other modification; blockade of the 3´ exonuclease activity
Modification structure	The amino group is bound to the 3´ end of the oligonucleotide by a 7-carbon spacer as standard. Spacers of different sizes are possible (can be ordered upon individual consultation).
Available purification types	OPC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 42 / oligo (VAT excluded)

TAMRA (3´ end)

Application	As a labelling molecule
Modification structure	Tetramethylrhodamin molecule is separated from the 3´ end of the oligonucleotide chain by a 7-carbon spacer
Available purification types	OPC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 71 / oligo (VAT excluded)

THIOL GROUP (3´ end)

Application	As an intermediate stage for further adjustments of the oligonucleotide (conjugation) while preserving the 5´ end for other modification; blockade of the 3´ exonuclease activity
Modification structure	Based on the deprotection method, either the bound thiol or disulphidic group is the result
Available purification types	OPC
Term of delivery	A, B
Extra fee for labelling for 200 nmol	EUR 38 / oligo (VAT excluded)