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Overview of Ribose Modifications
- 2´-amino-RNA, 2´-flouro-RNA (pure or mixed chains)
 | Modification structure | 2´ hydroxyl group of ribose is replaced with the primary amino group or fluorine, respectively. These modifications are even more stable against nucleases than 2´-O-methyl-RNA. Duplexes with RNA do not active RNase H and show even higher stability (higher Tm) than RNA/2´-O-methyl-RNA duplexes.
Pure as well as mixed chains can be prepared.
| | Application | Especially for antisense applications. | | Recommended purification type | OPC | | Term of delivery | X | | Price | Individual calculation is necessary (according to representation of individual bases) |
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- 2´-O-methyl-RNA (pure or mixed DNA/2´-O-methyl-RNA chains)
 | Modification structure | 2´ hydroxyl group of the ribose is methylated. Unlike RNA, 2´-O-methyl-RNA shows chemical stability (its stability against nucleases lasts days). Duplexes with RNA do not activate RNase H and are more stable (higher Tm) than DNA/RNA duplexes. Pure 2´-O-methyl-RNA: 2´-O-methyl-RNA is found at all positions in the sequence
Mixed DNA/2´-O-methyl-RNA chains: 2´-O-methyl-RNA is found only at selected positions in the sequence | | Application | 2´-O-methyl-RNA is used especially for antisense applications | | Recommended purification type | OPC | | Term of delivery | X | | Price | Individual calculation is necessary (according to representation of individual bases) | | Note: 2´-O-methyl-RNA with phosphorothioate modification of the chain and 2´-O-methyl-RNA with methylphosphonate modification can be produced upon consultation |
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