Overview of Modifications at the 5´ End

> Oligonucleotides > CATALOGUE AND PRICELIST > 5´ end modified oligos > Overview of Modifications at the 5´ End

Overview of Modifications at the 5´ End

Primary AMINO GROUP (5´ end)

Application	As an intermediate stage for further adjustments of the oligonucleotide (bond to special surfaces, conjugation)
Modification structure	Amino group bound to the 5´ end of the oligonucleotide chain by a 6-carbon spacer as standard - other ways of binding the amino group are possible upon individual consultation
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 93
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 93 within a single purchase order.

BIOTIN (5´ end)

Application	As a labelling molecule
Modification structure	-
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 280
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 280 within a single purchase order.

Cy3 (5´ end)

Application	As a cyanide dye
Characteristics	Cy3 is a protected trademark of Amersham
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 460
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 460 within a single purchase order.

Cy5 (5´ end)

Application	As a cyanide dye
Characteristics	Cy5 is a protected trademark of Amersham
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	460
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 460 within a single purchase order.

DABCYL (5´ end)

Application	As a labelling molecule; universal quencher
Modification structure	The molecule of 4-(dimethylamino)-azobenzene is bound to 5´ end of the oligonucleotide through the phosphate bond by an 8-atom spacer
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	X	MAX *200:**
Extra fee for the modification:	380
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 380 within a single purchase order.

DISULPHIDIC GROUP (5´ end)

Application	As an intermediate stage for further oligonucleotide modifications
Modification structure	The disulphidic group is bound to the 5´ end of the oligonucleotide chain by a 6-carbon spacer
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	X	MAX *200:**
Extra fee for the modification:	EUR 460
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 460 within a single purchase order.

DNP - dinitrophenyl (5´ end)

Application	As a labelling molecule (detection using the anti-DNP antibody)
Modification structure	DNP molecule is separated from the 5´ end of the oligonucleotide chain by a 15-atom spacer
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	X	MAX *200:**
Extra fee for the modification:	EUR 510
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 510 within a single purchase order.

6-FAM (5´ end)

Application	As fluorescein labelling molecule
Modification structure	Pure 6-carboxyfluorescein isomer
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 220
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 220 within a single purchase order.

FLUORESCEIN (5´ end)

Application	As fluorescein labelling molecule
Modification structure	Mixture of 5- and 6-carboxyfluorescein isomers
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 180
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 180 within a single purchase order.

PHOSPHORYLATION (5´ end)

Application	Usually for ligation that requires phosphorylation at the 5´ end of the chain
Modification structure	Chemical phosphorylation is more efficient than the enzymatic one.
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 170
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 170 within a single purchase order.

HEX (5´ end)

Application	As fluorescein labelling molecule
Modification structure	HEX is hexachlorofluorescein
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 170
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 170 within a single purchase order.

CHOLESTEROL (5´ end)

Application	To increase lipophilic characteristic of the oligonucleotide (in antisense oligonucleotides, to increase permeance of oligonucleotide chains through cellular membranes)
Modification structure	Cholesterol molecule is separated from the 5´ end of the oligonucleotide chain by a 15-atom spacer
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	X	MAX *200:**
Extra fee for the modification:	EUR 510
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 510 within a single purchase order.

TET (5´ end)

Application	As fluorescein labelling molecule
Modification structure	TET is tetrachlorofluorescein
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	X	MAX *200:**
Extra fee for the modification:	EUR 510
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 510 within a single purchase order.

THIOL GROUP (5´ end)

Application	As an intermediate stage for further oligonucleotide modifications (conjugation)
Modification structure	-
Recommended purification type:	OPC	*MAX 40:**
Term of delivery:	A, B	MAX *200:**
Extra fee for the modification:	EUR 170
* Up to 18 (MAX 40) labelled oligos in the scale 40 nmol or up to 10 (MAX 200) labelled oligos in the scale 200 nmol can be prepared for a single fee of EUR 170 within a single purchase order.