Oligos are synthesized with no additional charge when the degeneration is achieved by a simple mix of nucleotides in designated position. However, one exception exists: if degenerated position is located in the 3´end of oligo, the technique and price of a the synthesis will differ. In this case, it is necessary to manufacture a special column with an anchored mixture of nucleotides first. For this reason, when degeneration on the 3´end is ordered, it can be performed only at a scale of 200 nmol or higher and an additional fee is charged according to the table shown below.
When a small number of positions with complete degeneration (N=A, C, G, T) should be present in an oligonucleotide chain, degeneration can be achieved by simply mixing all four nucleotides in these positions. However, when a higher number of degenerate positions should be present in a chain, incorporation of 2'-deoxyinosine instead of N is highly advisable. Other possibility for degeneration is by using the universal nucleoside or dSpacer. Universal nucleoside does not have any ability to hybridize with any of the four nucleotides. Nitropyrrole or nitroindole are mostly used as universal nucleosides. dSpacer is a less costly possibility to make a position in an oligo chain that does not exhibit any ability of hybridization. dSpacer is a nucleoside not carrying any nucleotide.

DELIVERY TIMES FOR DEGENERATED OLIGONUCLEOTIDES

Degeneration using inosine

Inosine hybridizes to little extent with any of the four nucleotides. Use: at the place of "N" degeneration in the oligonucleotide chain, it can be incorporated at any position except for 3'end. Scales available: 40 nmol, 200 nmol, 1 micromol, 10 micromol. OPC purification: available. HPLC purification: available. Delivery time: A, B. Price: calculated as the price of an unmodified oligonucleotide at corresponding scale + additional charge for the degeneration procedure. MAX40: 18 MAX200: 10 Additional charge for degeneration procedure: 37 € Notes: "MAX40: 18" means that it is possible to incorporate 1 to 18 molecules of inosine to oligonucleotide chains at 40 nmol scale for the same additional charge 37 € "MAX200: 10" means that it is possible to incorporate 1 to 10 molecules of inosine to oligonucleotide chains at 200 nmol scale for the same additional charge 37 € Additional charge for a scale higher than 200 nmol can be calculated upon request.

 

Degeneration using the Universal nucleoside (nitropyrrole, nitroindole)

Universal nucleoside does not have any ability to hybridize with any of the four nucleotides. Use: Universal nusleoside can be incorporated at any position of the chain except for the 3'end. It is used instead of "N" positions in the chain. Scales available: 40 nmol, 200 nmol, 1 micromol, 10 micromol. OPC purification: available. HPLC purification: available. Delivery time: X. Price: calculated as the price of an unmodified oligonucleotide at corresponding scale + additional charge for the degeneration procedure. MAX40: 18 MAX200: 10 Additional charge for degeneration procedure: 296 € Notes: "MAX40: 18" means that it is possible to incorporate 1 to 18 molecules of Universal nucleoside to oligonucleotide chains at 40 nmol scale for the same additional charge 296 € "MAX200: 10" means that it is possible to incorporate 1 to 10 molecules of Universal nucleoside to oligonucleotide chains at 200 nmol scale for the same additional charge 296 € Additional charge for a scale higher than 200 nmol can be calculated upon request.

 

Degeneration using dSpacer

dSpacer is a nusleoside not carrying any nucleotide. Use: dSpacer can be incorporated at any position of the chain except for the 3'end. It is used instead of "N" positions in the chain. dSpacer is a non-nucleosidic building block (no nucleotide is bounded to deoxyribose). Scales available: 40 nmol, 200 nmol, 1 micromol, 10 micromol. OPC purification: available. HPLC purification: available. Delivery time: X. Price: calculated as the price of an unmodified oligonucleotide at corresponding scale + additional charge for the degeneration procedure. MAX40: 18 MAX200: 10 Additional charge for degeneration procedure: 205 € Notes: "MAX40: 18" means that it is possible to incorporate 1 to 18 molecules of dSpacer to oligonucleotide chains at 40 nmol scale for the same additional charge 205 € "MAX200: 10" means that it is possible to incorporate 1 to 10 molecules of dSpacer to oligonucleotide chains at 200 nmol scale for the same additional charge 205 € Additional charge for a scale higher than 200 nmol can be calculated upon request.