DNA/RNA integrity

Integrity of DNA/RNA is very important test needed according to the Europe pharmacopeia chapter 5.14.

Mostly the test can be done as agarose gel electrophoresis or using advanced techniques such as PCR or fragment analysis.

All the tests are according to the Europe pharmacopeia test 2.6.21.

To this test belong also plasmid DNA conformation test, because Stability of plasmid DNA depends on its conformation and the right conformation is required (e.g. supercoiled form).

For RNA integrity determination of RIN is important as well.

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gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.