Examples quantitative real-time PCR experiments performed by GB

Detection human mutation

• Deep vein thrombosis (FV Leiden, MTHFR C677T, MTHFR A1298C, Prothrombin II)
• Cystic fibrosis (Delta F508, G551D)
• Deletion in CX26 (deafness)
• MTR A66G, MTRR A2765G

Detection nonhuman mutation (veterinary)

• Scrapie mutations in PrP

Microbial detection

• Bioterrorism bacteria

Gene expression

• rat AhR
• rat biotransformation enzymes (CYP1A1, CYP3A4, CYP1B1, Carbonyl reductase, 11-beta-HSD type I, AKR1C9)
• human cyclooxygenases (COX1, COX2)
• human biotransformation enzymes (AKR1C1, AKR1C2, AKR1C3, AKR1C4)
• Human housekeeping (reference) gene
• RISE
• Human KRT1
• Human Mac-1
• Human apoptosis markers (P53, Bcl II, Bax, DFFA, DFFB, Casp3)
• Drug resistence related (MDR1, BCRP, MRP,…)