Generi Biotech - Compounds and Services for Molecular Biology

• Quantitative PCR
  • Sets for Gene Expression
  • qPCR sets for reference genes
  • Custom-Made qPCR Sets
  • Probe Master Mix
  • Standards for qPCR
  • Internal Positive Controls
• Sequencing
  • Standard Sequencing
  • 16S/ITS Sequencing
• Molecular Cloning
  • Custom cloning
  • Plasmid Amplification and Isolation
  • Competent Bacteria
• Cell Culture Applications
  • Recombinant Proteins
  • Recombinant Human SHBG
  • Cytotoxicity Testing
  • Luminescence Solution
  • Mycoplasma Detection
• Biological Identification
  • Cell Line Authentication
  • Paternity Outsourcing

16S/ITS Sequencing

One of possible ways of bacterial identification consists in sequencing of species-specific segments of genomic ribosomal DNA. These are 16S segments approximately 900 bases long and an "internal transcribed spacer" (ITS) approximately 600 bases long. The service includes amplification of these segments by the "proofreading" polymerase, isolation from the agarose gel and sequencing by specific primers.

Price

Terms of delivery

The results are sent via e-mail or by post on a CD within 10 working days upon receiving your order.

The customer obtains the sequences in the form of scf files with a transparent summary of the results arranged in a table (the so called sequencing key). Scf files can be viewed and modified using applications available for free download on the Internet, for example:

http://www.geospiza.com/Products/finchtv.shtml
http://www.technelysium.com.au/chromas_lite.html

How to place an order

Orders of bacteria identification using 16S/ITS sequencing can be sent to the e-mail address ; please enter SEQ keyword in the e-mail subject. It is also possible to print the completed purchase order form and send it by post together with the samples to our address.

For further information please contact us at the e-mail address above or at this phone number +420 495 056 394.

Requirements for the materials

Concentration of the bacterial DNA should be 100 ng/µl at the minimum and its minimum purity (A260/280) should be 1.8.