Universal Sequencing Primers for Standard Sequencing

NameSequenceApplication Examples
-47F (M13F)CGCCAGGGTTTTCCCAGTCACGACpUC19, pUC131, pCR-XL-TOPO, pCR4Blunt-TOPO, pCR4-TOPO, pGEM-3Zf(+)
alfaMFGAAGAAGCCTTGATTGGAalfa-Matting Factor (pKLAC1)
AOX3'CATCTCTCAGGCAAATGGCATTCTGAOX terminator (pGAPZaA)
AOX5'TTGCGACTGGTTCCAATTGACAAGAOX promotor
BGH_rTAGAAGGCACAGTCGAGGreverse primer for BGH polyA signal - Invitrogen (pZeoSV2(-))
CMV_-30GCCATCCACGCTGTTTTGACCTCCCMV promotor (pOptiVec, pZeoSV2(-))
CMV_-50TCAGATCGCCTGGAGACGCCATCCCMV promotor - distant (pOptiVec, pZeoSV2(-))
CMV_fCGCAAATGGGCGGTAGGCGTGCMV promotor forward primer - Invitrogen (pOptiVec, pZeoSV2(-), pLNCX2)
LAC4mtGCGGATAACAAGCTCAACLAC4 promotor (pKLAC1)
lambda gt10FCTTTTGAGCAAGTTCAGCCTGGTTAAGlambda phage derived vectors
lambda gt10RGAGGTGGCTTATGAGTATTTCTTCCAGGGTAlambda phage derived vectors
lambda gt11FGGTGGCGACGACTCCTGGAGCCCGlambda phage derived vectors
lambda gt11RTTGACACCAGACCAACTGGTAATGlambda phage derived vectors
M13 rev (vzdálený)CGTATGTTGTGTGGAATTGTGAGM13R distant primer (pCR4Blunt-TOPO, pCR4-TOPO, pCR-XL-TOPO, pGEM-3Zf(+), pOptiVec, pUC131)
M13Forward (-20)GTAAAACGACGGCCAGoficial M13 forward primer (pCR4Blunt-TOPO, pCR4-TOPO, pCR-XL-TOPO, pGEM-3Zf(+), pUC131)
M13ReverseCAGGAAACAGCTATGACoficial M13 reverse primer (pUC19, pUC131, pCR-XL-TOPO, pCR4Blunt-TOPO, pCR4-TOPO, pGEM-3Zf(+), pOptiVec)
malEGGTCGTCAGACTGTCGATGAAGCCpMAL vectors
p10SEQP_pQEGACCTTTAATTCAACCCAACACp10 promotor v pQE-TriSystem
pA_-120TGCAATTGTTGTTGTTAACTTGTTTATTGCAGCpolyA sequence
pet_rTTCACTTCTGAGTTCGGCATGreverse primer for pET vectors - Novagen
pGAP ForwardGTCCCTATTTCAATCAATTGAApGAPZaA
pGL_RVpr3CTAGCAAAATAGGCTGTCCC 
pGLpr2_RCTTTATGTTTTTGGCGTCTTCCA 
pKLAC1_rTTATCGCACAAGACAATCpKLAC1 reverse primer
pQE_FSGTTATTGTGCTGTCTCATCprimers for pQE-TriSystem
pQE_RSATCGATCTCAGTGGTATTTGTGprimers for pQE-TriSystem
puc_U1GGGGATGTGCTGCAAGGCGATTAApCR-XL-TOPO, pCR4Blunt-TOPO, pGEM-3Zf(+), pUC131
puc_U2CGGGCCTCTTCGCTATTACGCCAGpCR-XL-TOPO, pCR4Blunt-TOPO, pGEM-3Zf(+), pUC131
revers_ACGGCTCGTATGTTGTGTGGAATTGpCR4Blunt-TOPO, pCR4-TOPO, pCR-XL-TOPO, pGEM-3Zf(+), pOptiVec, pUC131
seq_IRES_tamCATGTGTTTAGTCGAGGTTApOptiVec
seq_IRES_zpetATATAGACAAACGCACACCGpOptiVec
seq_oriTTCCGCTTCCTCGCTCACTGpCR4Blunt-TOPO, pCR4-TOPO, pCR-XL-TOPO, pGEM-3Zf(+), pOptiVec, pET-DEST42, pET-15b, pUC131, pLNCX2
seq_PCICCACTCCCAGTTCAATTACAGCpCI
seq_pIRES-GCATTCTAGTTGTGGTTTGTCCpIRES, pZeoSV2(-)
seq_pIRES+GGCACCTATTGGTCTTACTGACpIRES
seq_pSecTag-ACAGTGGGAGTGGCACCTTCpSecTag2/Hygro vectors, pZeoSV2(-)
seq_pSecTag+CGGGACTTTCCAAAATGTCGpSecTag2/Hygro vectors, pOptiVec, pZeoSV2(-), pLNCX2
seq_retro_PSICCTCCTCTTCCTCCATCCGCsequence psi (pLNCX2)
SP6GATTTAGGTGACACTATAGSP6 promotor primer
T3-promATTAACCCTCACTAAAGGGAoficial T3 promotor primer (pCR4Blunt-TOPO, pCR4-TOPO)
T7-promTAATACGACTCACTATAGGGoficial T7 promotor primer (pCR4Blunt-TOPO, pCR4-TOPO, pCR-XL-TOPO, pGEM-3Zf(+), pET-DEST42, pET-15b, pZeoSV2(-))
T7-term_InvTAGTTATTGCTCAGCGGTGGT7 terminator reverse primer - Invitrogen (pET-DEST42, pET-15b)