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LIFE SCIENCES

Our product line Life Sciences comprises the most commonly used products for molecular biology (such as DNA isolation kits, PCR mastermixes, one-step reverse transcription kits etc.) including products specialized for real-time PCR. Our Life Sciences line comprises some services for R&D as well.

Our typical partners are end-users of the products (customers mostly in CZ and SK), business partners and distributors. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, to their sales strategy (see here).

Authentication of cell lines

Authentication of cell lines Authentication of cell lines

Using a forensic kit, selected autosomal polymorphisms are amplified in a multiplex PCR. The polymorphic fragments of DNA are then separated in capillary electrophoresis. The same method is applied as well in forensic testing.

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Human Cell Line Authentication on LabCard

Human Cell Line Authentication on LabCard Human Cell Line Authentication on LabCard

LabCards are intended for simple transport of a cell sample or genomic DNA of human cell lines. Samples applied to the LabCard do not need to be transported chilled. STR analysis is performed as part of the service.

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Plasmid amplification and isolation

Plasmid amplification and isolation Plasmid amplification and isolation

Plasmid amplification is provided in Escherichia coli bacteria cells. Plasmid linearization by restriction cleavage can be ordered as a follow-up service. Such an operation is recommended especially when the plasmid is used as a PCR standard. Amplified plasmids are delivered either in midiprep or maxiprep quantities. Typical yields are stated below.

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Plasmid linearization

Plasmid linearization Plasmid linearization

If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.

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Plasmid verification

Plasmid verification Plasmid verification

This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.

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Long-term storage of bacterial stocks

Long-term storage of bacterial stocks Long-term storage of bacterial stocks

The service is complementary for plasmids prepared in GENERI BIOTECH. It includes storage of a bacterial culture containing the plasmid. The standard storage period is one year.

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Mycoplasma detection – qualitative PCR

Mycoplasma detection – qualitative PCR Mycoplasma detection – qualitative PCR

The method involves isolating DNA from a sample of a conditioned eukaryotic cell medium. Subsequently, amplification and detection of a highly conserved region encoding 16S ribosomal RNA specific for mycoplasmas is performed by real-time PCR. This method is used for the qualitative determination of mycoplasmas in a sample.

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