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LIFE SCIENCES

Our product line Life Sciences comprises  oligonucleotides in many categories, the most commonly used products for molecular biology (such as DNA isolation kits, PCR mastermixes, reverse transcription kits etc.) including products specialized for real-time PCR (assays for reference genes, assays on demand etc.).

Our Life Sciences line comprises some services for R&D as well.

Our typical partners are end-users of the products (customers mostly in CZ and SK), business partners and distributors mostly within EU. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, to their sales strategy (see here).

Authentication of cell lines

Authentication of cell lines Authentication of cell lines

Using a forensic kit, selected autosomal polymorphisms are amplified in a multiplex PCR. The polymorphic fragments of DNA are then separated in capillary electrophoresis. The same method is applied as well in forensic testing.

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Plasmid amplification and isolation

Plasmid amplification and isolation Plasmid amplification and isolation

Plasmid amplification is provided in Escherichia coli bacteria cells. Plasmid linearization by restriction cleavage can be ordered as a follow-up service. Such an operation is recommended especially when the plasmid is used as a PCR standard. Amplified plasmids are delivered either in midiprep or maxiprep quantities. Typical yields are stated below.

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Plasmid linearization

Plasmid linearization Plasmid linearization

If the plasmid DNA is intended for use as a PCR template, it is recommended to use it as a linear DNA. A circular plasmid mostly has a supercoiled conformation, where the target sequence is less accessible for primers and for polymerase.

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Plasmid verification

Plasmid verification Plasmid verification

This service is recommended when it is necessary to verify whether a correct sequence was cloned in, whether plasmids were not misidentified (mistaken by accident), or to verify that there are no errors in the plasmid sequence.

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Long-term storage of bacterial stocks

Long-term storage of bacterial stocks Long-term storage of bacterial stocks

The service is complementary for plasmids prepared in GENERI BIOTECH. It includes storage of a bacterial culture containing the plasmid. The standard storage period is one year.

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qPCR assays for human reference genes

qPCR assays for human reference genes qPCR assays for human reference genes

The product is a lyophilized mixture of 2 primers and a hydrolysis probe. In combination with the reaction mixture (gb Master Mix according to your own choice or another reaction mixture of the customer) it is used for qPCR. By default, it is possible to order sets for human, rat and mouse housekeeping genes.

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qPCR assays for mouse reference genes

qPCR assays for mouse reference genes qPCR assays for mouse reference genes

The product is a lyophilized mixture of 2 primers and a hydrolysis probe. In combination with the reaction mixture (gb Master Mix according to your own choice or another reaction mixture of the customer) it is used for qPCR. By default, it is possible to order sets for human, rat and mouse housekeeping genes.

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qPCR assays for rat reference genes

qPCR assays for rat reference genes qPCR assays for rat reference genes

The product is a lyophilized mixture of 2 primers and a hydrolysis probe. In combination with the reaction mixture (gb Master Mix according to your own choice or another reaction mixture of the customer) it is used for qPCR. By default, it is possible to order sets for human, rat and mouse housekeeping genes.

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Customized High-Throughput PCR Array

Customized High-Throughput PCR Array Customized High-Throughput PCR Array

Oligos on array are recommended when high number of PCR / real-time PCR tests are needed to be performed paralelly on one microplate. After adding MasterMix + DNA sample, the plates are ready for PCR / real-time PCR. Saving time is the main benefit of this service. Resulting arrays contain all compounds to provide either PCR or real-time PCR according to customer´s design.

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Ready-to-use High-Throughput PCR Array

Ready-to-use High-Throughput PCR Array Ready-to-use High-Throughput PCR Array

Oligos + MasterMix on array are recommended when high number of PCR / real-time PCR tests are needed to be performed paralelly on one microplate. After adding sample DNA the plates are ready for PCR/real-time PCR. Considerable time saving is the main benefit of this service. The resulting arrays contain all compounds to provide either PCR or real-time PCR according to customers´ design.

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