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modified oligonucleotides

modified oligonucleotides

The most common modifications are at the end of the string – at the 3’ or 5’ end, or combined. The modifying molecule may be in the middle of the chain, the bases in the chain may be replaced by their analogs; spacers of different lengths and nature, functional groups or molecules may be inserted into the chain. Modified phosphate linkage may be in the chain in all or only in selected positions. Less frequent modifications or their combinations are recommended to be consulted first because there are many limitations for synthesis and for purification of the resulting product.

8-Oxo-dG

8-Oxo-dG 8-Oxo-dG

May be attached anywhere within the oligonucleotide sequence except for the 3' end. It is used for structural and activity studies concerning oligonucleotides carrying an 8-oxo mutation. This naturally occurs when DNA is exposed to oxidative agents or ionizing radiation.

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Amino C3sp

Amino C3sp Amino C3sp

The amino group is bound to the 5' end of the oligonucleotide sequence by a three carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation).

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Amino C6sp

Amino C6sp Amino C6sp

The amino group is bound to the 5' end of the oligonucleotide sequence by a six carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation).

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Amino C6sp-dA

Amino C6sp-dA Amino C6sp-dA

May be attached anywhere within the oligonucleotide sequence except for the 3' end. The primary amino group is bound by a spacer to the purine circle of adenosine.

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Amino C6sp-dC

Amino C6sp-dC Amino C6sp-dC

May be attached anywhere within the oligonucleotide sequence except for the 3' end. The primary amino group is bound by a spacer to the pyrimidine circle of cytosine.

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Amino C6sp-dT

Amino C6sp-dT Amino C6sp-dT

May be attached anywhere within the oligonucleotide sequence except for the 3' end. The primary amino group is bound by a spacer to the pyrimidine circle of thymine.

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Amino C7sp

Amino C7sp Amino C7sp

The amino group is bound to the 3' end of the oligonucleotide sequence by a seven carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation) while keeping the 5' end free for another possible modification and as a blocker of 3' polymerase extension.

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Amino N2-C6sp-dG

Amino N2-C6sp-dG Amino N2-C6sp-dG

May be attached anywhere within the oligonucleotide sequence except for the 3' end. The primary amino group is bound by a spacer to the purine circle of guanosine.

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BBQ650

BBQ650 BBQ650

Quencher suitable for fluorophores having an emission wavelength range from 550 to 750 nm. BBQ-650 may be attached to the 5'end or the 3' end of the oligonucleotide.

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BHQ1

BHQ1 BHQ1

A quencher suitable for fluorophores having an emission wavelength range from 480 to 580 nm. BHQ1 may be attached anywhere within the oligonucleotide sequence.

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BHQ2

BHQ2 BHQ2

A quencher suitable for fluorophores having an emission wavelength range from 540 to 670 nm. BHQ2 may be attached anywhere within the oligonucleotide sequence.

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BHQ3

BHQ3 BHQ3

Quencher suitable for fluorophores having an emission wavelength range from 620 to 730 nm. BHQ3 may be attached to the 3'end of the oligonucleotide.

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