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OLIGO & PROBES

We are an EN ISO 9001, 13485 and 17025 certified EU-located company manufacturing custom DNA oligos. We have been synthesizing custom DNA oligonucleotides since 1995. Every year our custom oligo synthesis services produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications and for various purposes (biological techniques based mostly on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

The know-how we acquired in custom DNA oligo synthesis during the last 24 years is implemented into the development of other products, especially CE IVD kits and Life Sciences products.

Our typical partners are end-users of the custom DNA oligos (customers mostly in CZ and SK), business partners and distributors mostly within EU. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, into their sales strategy.

3-Deaza-dA

3-Deaza-dA 3-Deaza-dA

It is used to study oligonucleotide activity changes following alteration of the main structural component. 3-Deaza-dA may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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5-(1-Propynyl)-dC (pdC)

5-(1-Propynyl)-dC (pdC) 5-(1-Propynyl)-dC (pdC)

It is used to increase duplex stability and melting temperature while preserving specificity of hybridization. 5-(1-Propynyl)-dC may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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5-(1-Propynyl)-dU (pdU)

5-(1-Propynyl)-dU (pdU) 5-(1-Propynyl)-dU (pdU)

It is used to increase duplex stability and melting temperature while preserving specificity of hybridization. 5-(1-Propynyl)-dU may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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Biotin-TEG

Biotin-TEG Biotin-TEG

Biotin is ligated to the oligonucleotide by means of a triethylene glycol spacer (TEG), extending the distance between the oligonucleotide and the biotin by 15 atoms. Biotin-TEG may be ligated to the 3 'end of the oligonucleotide.

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ROX

ROX ROX

ROX (6-Carboxy-X-Rhodamine) is a red fluorophore. It can be linked to the 3 'end of the chain, or by using the NHS ester at 5'. In case of binding at the 5 'end, the amino linker is first attached to the oligonucleotide through which the post-synthetic binding of ROX then proceeds.

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Spermine

Spermine Spermine

It is used for manufacturing of oligospermine-oligonucleotide conjugates (Zip Nucleic Acid - ZNA Oligos). ZNA oligos are employed e.g. in multiplex PCR, PCR of AT-rich sequences, RT qPCR, miRNA detection and improving SNP discrimination. Spermine may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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N4-Et-dC

N4-Et-dC N4-Et-dC

It serves as a substitute for dC in GC-rich sequences in order to prevent the formation of oligonucleotide secondary structures because N4-Et-dC specifically hybridizes with natural dG. N4-Et-dC may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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Cholesterol

Cholesterol Cholesterol

Adding cholesterol increases oligonucleotide lipophility. Furthermore, cholesterol improves the permeability of oligonucleotide chains through cellular membranes in antisense oligonucleotides (phosphorothioates or other derivatives). Cholesterol may be attached either to the 5' end or the 3' end of the oligonucleotide.

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DNP

DNP DNP

DNP is a labelling molecule detectable by an anti-DNP antibody. DNP may be attached to the 5' end of the oligonucleotide. The dinitrophenyl molecule is separated from the 5' end of the oligonucleotide by a fifteen carbon spacer.

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dU

dU dU

Deoxyuridine is usually introduced to the oligonucleotide DNA instead of deoxythimine. May be attached anywhere within the oligonucleotide sequence.

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8-Br-dG

8-Br-dG 8-Br-dG

May be attached anywhere within the oligonucleotide sequence except for the 3' end. It is used for crystallographic studies of oligonucleotide structures. 8-Br-dG is photolabile, the feature which is utilized in cross-linking studies investigating protein-DNA complex structures.

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8-Br-dA

8-Br-dA 8-Br-dA

May be attached anywhere within the oligonucleotide sequence except for the 3' end. It is used for crystallographic studies of oligonucleotide structures. 8-Br-dA is photolabile, the feature which is utilized in cross-linking studies investigating protein-DNA complex structures.

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