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OLIGO & PROBES

We are an EN ISO 9001 and 13485 certified EU-located company manufacturing custom DNA oligos. We have been synthesizing custom DNA oligonucleotides since 1995. Every year our custom oligo synthesis services produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications and for various purposes (biological techniques based mostly on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

The know-how we acquired in custom DNA oligo synthesis during more than 25 years is implemented into the development of other products, especially CE IVD kits and Life Sciences products.

Our typical partners are end-users of the custom DNA oligos (customers mostly in CZ and SK), business partners and distributors. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, into their sales strategy.

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Tiles List

Probe FRET 5´CFR 635

The FRET 5´CFR 635 probe is a hybridization real-time PCR probe labeled at the 5´ end of the sequence with the fluorophore CFR 635. It enables specific detection of target DNA and SNP genotyping. It is used as an acceptor probe in combination with the FRET 3´FAM donor probe.

in-stock SEQplus universal sequencing primers

In addition to custom synthesis of 5’SEQplus modified sequencing primers, we hold some frequently used 5’SEQplus modified universal sequencing primers in stock (HPLC purified).

NGS Primers

Introducing our cutting-edge NGS oligonucleotide purification technology that delivers exceptional purity and minimizes cross-contamination. Our innovative methodology is designed to meet the demanding requirements of next-generation sequencing laboratories, ensuring maximum accuracy and reliability of your results.

oligo DUPLEX

We can prepare double-stranded DNA (oligo DUPLEX) from complementary single-stranded oligonucleotides (ssDNA). Duplex can be prepared from two oligonucleotides ordered by you, or we can design the complementary strand to the oligo sequence supplied by you.

Probe FRET 3´FAM

The FRET 3´FAM probe is a hybridization real-time PCR probe labeled with the fluorophore 6-FAM at the 3’ end of the sequence. It enables specific detection of target DNA and SNP genotyping. It is used as a donor probe in combination with an acceptor probe from the CFR series.

Inosin

2'deoxyinosin is a "universal" base (it has the ability to bind to each of the 4 standard bases). It can be located anywhere in the oligonucleotide chain.

2’F-RNA

2'-F-RNA is even more stable than 2'-O-methyl-RNA. 2'-F-RNA & RNA duplexes do not activate RNase H and are more stable (with higher Tm) than RNA & 2'-O-methyl-RNA duplexes. It serves mainly for antisense applications. May be attached anywhere within the oligonucleotide sequence.

2’OMe-RNA

Compared to RNA 2'-O-methyl-RNA is more chemically stable (being stable against nucleases for several days). 2'-O-methyl-RNA & RNA duplexes do not activate RNase H and are more stable (with higher Tm) than DNA & RNA duplexes. It is used mainly in antisense applications. May be attached anywhere within the oligonucleotide sequence.

2′3′-ddC

It serves as a blocker of 3' polymerase extension. 2',3'-ddC may be attached to the 3' end of the oligonucleotide.

BiotinTEG

Biotin is ligated to the oligonucleotide by means of a triethylene glycol spacer (TEG), extending the distance between the oligonucleotide and the biotin by 15 atoms. Biotin-TEG may be ligated to the 3 'end of the oligonucleotide.

ROX

ROX (6-Carboxy-X-Rhodamine) is a red fluorophore. It can be linked to the 3 'end of the chain, or by using the NHS ester at 5'. In case of binding at the 5 'end, the amino linker is first attached to the oligonucleotide through which the post-synthetic binding of ROX then proceeds.

Cholesterol

Adding cholesterol increases oligonucleotide lipophility. Furthermore, cholesterol improves the permeability of oligonucleotide chains through cellular membranes in antisense oligonucleotides (phosphorothioates or other derivatives). Cholesterol may be attached either to the 5' end or the 3' end of the oligonucleotide.

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