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OLIGO & PROBES

We are an EN ISO 9001 and 13485 certified EU-located company manufacturing custom DNA oligos. We have been synthesizing custom DNA oligonucleotides since 1995. Every year our custom oligo synthesis services produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications and for various purposes (biological techniques based mostly on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

The know-how we acquired in custom DNA oligo synthesis during more than 25 years is implemented into the development of other products, especially CE IVD kits and Life Sciences products.

Our typical partners are end-users of the custom DNA oligos (customers mostly in CZ and SK), business partners and distributors. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, into their sales strategy.

BHQ2

BHQ2 BHQ2

A quencher suitable for fluorophores having an emission wavelength range from 540 to 670 nm. BHQ2 may be attached anywhere within the oligonucleotide sequence.

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BHQ1

BHQ1 BHQ1

A quencher suitable for fluorophores having an emission wavelength range from 480 to 580 nm. BHQ1 may be attached anywhere within the oligonucleotide sequence.

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Cy3.5

Cy3.5 Cy3.5

May be attached anywhere within the oligonucleotide sequence except for the 3' end.

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Yakima Yellow

Yakima Yellow Yakima Yellow

Yakima Yellow may be attached either to the 5' end or the 3' end of the oligonucleotide.

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JOE

JOE JOE

6-karboxy-4', 5'-dichlor-2', 7'-dimethoxyfluorescein is commonly used in multiplex reactions together with FAM. JOE may be attached to the 5' end of the oligonucleotide.

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Thiol S-S

Thiol S-S Thiol S-S

It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation). Thiol S-S may be attached to the 5' end of the oligonucleotide.

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Thiol SH

Thiol SH Thiol SH

It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation) and as a blocker of 3' polymerase extension. Thiol SH may be attached either to the 5' end or the 3' end of the oligonucleotide.

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CFR 635

CFR 635 CFR 635

CFR 635 is an orange-red fluorophore. It is used as a good alternative to LC red 640. CFR 635 may be attached only to 5´end of an oligonucleotide.

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Biotin

Biotin Biotin

Biotin may be attached either to the 5' end or the 3' end of the oligonucleotide. The biotin molecule is separated from the 3' end by a sixteen carbon spacer.

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Cy5

Cy5 Cy5

May be attached to the 5' end of the oligonucleotide. Oligonucleotide purification is a necessity.

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Cy3

Cy3 Cy3

May be attached anywhere within the oligonucleotide sequence except for the 3' end.

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TET

TET TET

Tetrachlorofluorescein is commonly used for multiplex reactions with FAM and HEX. It can be linked to an oligonucleotide at the 5 'end.

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