We are an EN ISO 9001 and 13485 certified EU-located company manufacturing custom DNA oligos. We have been synthesizing custom DNA oligonucleotides since 1995. Every year our custom oligo synthesis services produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications and for various purposes (biological techniques based mostly on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.
The know-how we acquired in custom DNA oligo synthesis during more than 25 years is implemented into the development of other products, especially CE IVD kits and Life Sciences products.
Our typical partners are end-users of the custom DNA oligos (customers mostly in CZ and SK), business partners and distributors. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, into their sales strategy.
HEX Hexachlorofluorescein is commonly used in multiplex reactions together with FAM and TET. Hexachlorofluorescein may be attached to the 5' end of the oligonucleotide.
Fluorescein-dT Fluorescein dT is attached by a six carbon spacer at position 5 of the thymine circle. Fluorescein dT may be attached anywhere within the oligonucleotide sequence.
Fluorescein It is a mixture of 5- and 6-carboxyfluorescein isomers. Fluorescein may be attached anywhere within the oligonucleotide sequence.
6-FAM Denotes pure 6-carboxyfluorescein isomer. This is among the most used oligonucleotide fluorescent modifications and is compatible with the majority of fluorescent devices. 6-FAM may be attached either to the 5' end or the 3' end of the oligonucleotide.
CFR 610 CFR 610 is an orange-red fluorophore. It is also used as a good alternative to fluorophore Texas Red. CFR 610 may be attached only to 5´end of an oligonucleotide.
Amino C6sp The amino group is bound to the 5' end of the oligonucleotide sequence by a six carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation).
Spacer C3 May be attached anywhere within the oligonucleotide sequence. Repeated spacer attachment is possible, forming a long hydrophobic arm. When bound to the 3' end it serves as a blocker of 3' polymerase extension.
Amino C3sp The amino group is bound to the 5' end of the oligonucleotide sequence by a three carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation).
Amino C7sp The amino group is bound to the 3' end of the oligonucleotide sequence by a seven carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation) while keeping the 5' end free for another possible modification and as a blocker of 3' polymerase extension.
Cy5.5 It is a deep red fluorophore. May be attached to the 5‘ end of the oligonucleotide. Oligonucleotide purification is a necessity.
in-stock oligonucleotides The most frequently entered sequences are prepared as a finished product in the warehouse. The advantage of storing oligo products is cleaning, which is not necessary to enter and pay separately.
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