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OLIGO & PROBES

We are an EN ISO 9001 and 13485 certified EU-located company manufacturing custom DNA oligos. We have been synthesizing custom DNA oligonucleotides since 1995. Every year our custom oligo synthesis services produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications and for various purposes (biological techniques based mostly on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

The know-how we acquired in custom DNA oligo synthesis during more than 25 years is implemented into the development of other products, especially CE IVD kits and Life Sciences products.

Our typical partners are end-users of the custom DNA oligos (customers mostly in CZ and SK), business partners and distributors. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, into their sales strategy.

Position of modification

Tiles List

CFR 610

CFR 610 is an orange-red fluorophore. It is also used as a good alternative to fluorophore Texas Red. CFR 610 may be attached only to 5´end of an oligonucleotide.

Amino C6sp

The amino group is bound to the 5' end of the oligonucleotide sequence by a six carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation).

Spacer 9

May be attached anywhere within the oligonucleotide sequence except for the 3' end.

Spacer C3

May be attached anywhere within the oligonucleotide sequence. Repeated spacer attachment is possible, forming a long hydrophobic arm. When bound to the 3' end it serves as a blocker of 3' polymerase extension.

Amino C3Sp

The amino group is bound to the 5' end of the oligonucleotide sequence by a three carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation).

Amino C7sp

The amino group is bound to the 3' end of the oligonucleotide sequence by a seven carbon spacer. It is used as an intermediate for further oligo modifications (attachment to special surfaces, conjugation) while keeping the 5' end free for another possible modification and as a blocker of 3' polymerase extension.

Cy5.5

It is a deep red fluorophore. May be attached to the 5‘ end of the oligonucleotide. Oligonucleotide purification is a necessity.

in-stock oligonucleotides

The most frequently entered sequences are prepared as a finished product in the warehouse. The advantage of storing oligo products is cleaning, which is not necessary to enter and pay separately.

ATTO dyes

ATTO dyes are a series of fluorescent dyes which provide all the crucial properties required for modern fluorescent technologies with spectral region from 350 nm in the UV to 750 nm in the near infrared.

Probe GEMINI™ Cy5

GEMINI™ Cy5 is a highly specific hydrolysis real-time PCR probe labeled with Cy5. It provides accurate detection of the target sequence with low background and high sensitivity due to the use of an internal quencher, especially for longer AT-rich probes. Suitable for gene expression and diagnostic applications.

Probe Cy5-BBQ650

Cy5-BBQ650 is a real-time PCR dual-labeled probe with Cy5 fluorophore and BBQ650 dark quencher. It can be used as an alternative to BHQ2 quencher due to its broader absorption spectrum and higher stability. The probe is most commonly used as a Taqman-type hydrolysis probe providing highly sensitive and specific detection of target DNA.

Probe HEX-BBQ650

HEX-BBQ650 is a real-time PCR dual-labeled probe with HEX fluorophore and BBQ650 dark quencher. It can be used as an alternative to BHQ2 quencher due to its broader absorption spectrum and higher stability. The probe is most commonly used as a Taqman-type hydrolysis probe providing highly sensitive and specific detection of target DNA.

Position of modification