RT PCR probes FRET

RT-PCR FRET probes enable sensitive and specific qPCR detection using a donor–acceptor pair and Förster resonance energy transfer.
Our probes are manufactured to high purity and are suitable for SNP genotyping and other real-time PCR applications. Custom synthesis and labeling upon request.

Popular FRET probe pairs

FRET 3´FAM (donor) – 3’ end probe with 6-FAM donor fluorophore.
FRET 5´CFR 610 (acceptor) – 5’ end probe with CFR 610, pairs with 3´FAM.
FRET 5´CFR 635 (acceptor) – 5’ end probe with CFR 635, pairs with 3´FAM.

Looking for other probe chemistries? See
RT-PCR Dual-Labeled Probes
or explore all oligonucleotides.
For tailored designs and multiplexing advice, contact our team.

Tiles List

Probe FRET 3´FAM

The FRET 3´FAM probe is a hybridization real-time PCR probe labeled with the fluorophore 6-FAM at the 3’ end of the sequence. It enables specific detection of target DNA and SNP genotyping. It is used as a donor probe in combination with an acceptor probe from the CFR series.

Probe FRET 5´CFR 610

The FRET 5´CFR 610 probe is a hybridization real-time PCR probe labeled at the 5’ end of the sequence with the fluorophore CFR 610. It enables specific detection of target DNA and SNP genotyping. It is used as an acceptor probe in combination with the FRET 3´FAM donor probe.

Probe FRET 5´CFR 635

The FRET 5´CFR 635 probe is a hybridization real-time PCR probe labeled at the 5´ end of the sequence with the fluorophore CFR 635. It enables specific detection of target DNA and SNP genotyping. It is used as an acceptor probe in combination with the FRET 3´FAM donor probe.