About oligos

In modern molecular biology and clinical diagnostics, fluorescently labeled oligonucleotide probes constitute a crucial component of nucleic acid detection methods. While “native” dyes (e.g., those from the fluorescein or rhodamine families) have been historically established in standard protocols, recent advances in chemical synthesis provide a broad spectrum of alternative fluorophores that offer equivalent, and in

Standard oligonucleotides reliably cover routine laboratory needs. However, more complex experimental designs—such as the preparation of long control templates, specific probes, or advanced genetic manipulations—often require sequences exceeding one hundred bases. To address these specific research applications, GENERI BIOTECH has expanded its portfolio with a new offering: ultramers. Standard Synthesis Parameters Ultramers are a targeted

Backbone modifications alter the fundamental sugar–phosphate structure of an oligonucleotide. They are used to enhance stability, nuclease resistance, and binding affinity, making oligos suitable for demanding biological and therapeutic applications. Backbone modifications refer to chemical changes that affect the sugar–phosphate backbone of an oligonucleotide. These modifications alter the fundamental structure of the oligo rather than

No more waiting for the certificate to arrive by email — now you can download it anytime with just one click. We have introduced a new feature for instant generation of the CoA (Certificate of Analysis) for oligonucleotides directly in your customer account. What does the new feature allow? Generate a CoA for the entire

A universal base is a modified nucleobase that can pair (non-selectively) with more than one of the standard DNA bases (A, T, G, C), ideally without significantly destabilizing the duplex or interfering with polymerase activity. Inosine is the most common universal base, although other possibilities exist: Common Universal Bases in Oligonucleotide Synthesis Name Description Base-Pairing

The degenerated base “N” and the universal base inosine (I) both serve to introduce base ambiguity in oligonucleotide synthesis, but they do so in different ways and are used in different contexts. Here’s a comparison: Inosine (I) vs. Degenerate Base “N” Feature Inosine (I) Degenerate Base “N” Chemical identity A real, single modified nucleotide A

Degen bases like dK and dP refer to synthetic, non-standard nucleobases used in molecular biology used in oligonucleotide synthesis — to allow degenerate (ambiguous) base pairing. They are alternatives to the classic “N” base, used to encode a mixture of A, T, G, C at a given position, but with greater control and performance in

Definition and Characteristics of Analog Bases Analog bases are chemically modified variants of the natural nucleotides—adenine (A), cytosine (C), guanine (G), and thymine (T)—that retain their ability to form complementary base pairs while exhibiting distinct chemical and structural properties. Within sequencing workflows, such modifications can minimize amplification bias, improve detection through enhanced fluorescent signaling, or

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.