Array I layout
plate | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
A | DNMT3B | PODXL | ZFP42 | ACTC1 | BRIX1 | CD9 | CDH1 | FGF4 | FOXD3 | GABRB3 | GRB7 | LEFTY1 |
B | NODAL | REST | RUNX2 | TERT | ALDH1A1 | ALDH2 | APC | CDH2 | FGFR1 | HDAC2 | KAT2A | KAT7 |
C | KAT8 | PARD6A | COMMD3 | IL6ST | LIFR | SFRP2 | OCT4 | SOX2 | KLF4 | LIN28A | NANOG | NR5A2 |
D | UTF1 | ALPL | GDF3 | TDGF1 | CCNA2 | CDK1 | CDC42 | DPPA2 | DPPA3 | FGF2 | HSPA9 | MYBL2 |
E | OTX2 | SOX15 | TBX3 | TCF3 | CD34 | GJB2 | KRT15 | COL1A1 | COL2A1 | EMX2 | FABP7 | NCAM1 |
F | NES | NUMB | OLIG2 | PECAM1 | SYCP3 | PAX6 | TUBB3 | FGF8 | FN1 | GFAP | NEUROD1 | TH |
G | BMP4 | GATA2 | HAND1 | MESP1 | HBB | RUNX1 | FOXA2 | GATA4 | HHEX | HNF4A | SOX17 | EOMES |
H | KLF2 | TFCP2L1 | DNMT1 | DNMT3A | DNMT3L | XIST | GAPDH | B2M | SDHA | HPRT1 | NO-RT control | PCR positive control |
Cartilage | Neuronal Stem Cell | Cardiomyocyte | Germ cells | Other Reprogramming Factors | Mesoderm | Endoderm | Naive gene markers | House keeping genes |
iPSC Markers | Other iPSC-Related Genes | Reprogramming Factors | Ectoderm | Reprogramming Enhancers & Inhibitors | ESC Markers | Other ESC-Related Genes | Keratinocyte | Blood Stem Cell |
list of genes
Function | # | Array position |
Gene Symbol | Gene Name | Description | GeneBank |
iPSC Markers | 1 | A1 | DNMT3B | ICF, ICF1, M.HsaIIIB | DNA (cytosine-5-)-methyltransferase 3 beta | NM_006892 |
2 | A2 | PODXL | Gp200, PC, PCLP, PCLP-1 | Podocalyxin-like | NM_005397 | |
3 | A3 | ZFP42 | REX1, ZNF754 | Zinc finger protein 42 homolog (mouse) | NM_174900 | |
Other iPSC-Related Genes | 4 | A4 | ACTC1 | ACTC, ASD5, CMD1R, CMH11, LVNC4 | Actin, alpha, cardiac muscle 1 | NM_005159 |
5 | A5 | BRIX1 (BXDC2) | BRIX, BXDC2 | BRX1, biogenesis of ribosomes, homolog (S. cerevisiae) | NM_018321 | |
6 | A6 | CD9 | BTCC-1, DRAP-27, MIC3, MRP-1, TSPAN-29, TSPAN29 | CD9 molecule | NM_001769 | |
7 | A7 | CDH1 | Arc-1, CD324, CDHE, ECAD, LCAM, UVO | Cadherin 1, type 1, E-cadherin (epithelial) | NM_004360 | |
8 | A8 | FGF4 | HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF | Fibroblast growth factor 4 | NM_002007 | |
9 | A9 | FOXD3 | AIS1, Genesis, HFH2, VAMAS2 | Forkhead box D3 | NM_012183 | |
10 | A10 | GABRB3 | ECA5 | Gamma-aminobutyric acid (GABA) A receptor, beta 3 | NM_000814 | |
11 | A11 | GRB7 | – | Growth factor receptor-bound protein 7 | NM_005310 | |
12 | A12 | LEFTY1 | LEFTB, LEFTYB | Left-right determination factor 1 | NM_020997 | |
13 | B1 | NODAL | HTX5 | Nodal homolog (mouse) | NM_018055 | |
14 | B2 | REST | NRSF, XBR | RE1-silencing transcription factor | NM_005612 | |
15 | B3 | RUNX2 | AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA | Runt-related transcription factor 2 | NM_004348 | |
16 | B4 | TERT | CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT | Telomerase reverse transcriptase | NM_198253 | |
17 | B5 | ALDH1A1 | ALDC, ALDH-E1, ALDH1, ALDH11, HEL-S-53e, HEL12, PUMB1, RALDH1 | Aldehyde dehydrogenase 1 family, member A1 | NM_000689 | |
18 | B6 | ALDH2 | ALDH-E2, ALDHI, ALDM | Aldehyde dehydrogenase 2 family (mitochondrial) | NM_000690 | |
19 | B7 | APC | BTPS2, DP2, DP2.5, DP3, GS, PPP1R46 | Adenomatous polyposis coli | NM_000038 | |
20 | B8 | CDH2 | CD325, CDHN, CDw325, NCAD | Cadherin 2, type 1, N-cadherin (neuronal) | NM_001792 | |
21 | B9 | FGFR1 | BFGFR, CD331, CEK, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1 | Fibroblast growth factor receptor 1 | NM_015850 | |
22 | B10 | HDAC2 | HD2, RPD3, YAF1 | Histone deacetylase 2 | NM_001527 | |
23 | B11 | KAT2A | GCN5, GCN5L2, PCAF-b, hGCN5 | K(lysine) acetyltransferase 2A | NM_021078 | |
24 | B12 | KAT7 | HBO1, HBOA, MYST2, ZC2HC7 | K(lysine) acetyltransferase 7 | NM_007067 | |
25 | C1 | KAT8 | MOF, MYST1, ZC2HC8, hMOF | K(lysine) acetyltransferase 8 | NM_032188 | |
26 | C2 | PARD6A | PAR-6A, PAR6, PAR6C, PAR6alpha, TAX40, TIP-40 | Par-6 partitioning defective 6 homolog alpha (C. elegans) | NM_016948 | |
27 | C3 | COMMD3 | BUP, C10orf8, RP11-573G6.3 | COMM domain containing 3 | NM_012071 | |
28 | C4 | IL6ST | CD130, CDW130, GP130, IL-6RB | interleukin 6 signal transducer (gp130, oncostatin M receptor) | NM_175767 | |
29 | C5 | LIFR | CD118, LIF-R, SJS2, STWS, SWS | leukemia inhibitory factor receptor alpha | NM_002310 | |
30 | C6 | SFRP2 | FKSG12, FRP-2, SARP1, SDF-5 | secreted frizzled-related protein 2 | NM_003013 | |
Reprogramming Factors | 31 | C7 | POU5F1 (Oct3/4) | OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4 | POU class 5 homeobox 1 | NM_002701 |
32 | C8 | SOX2 | ANOP3, MCOPS3 | SRY (sex determining region Y)-box 2 | NM_003106 | |
33 | C9 | KLF4 | EZF, GKLF | Kruppel-like factor 4 (gut) | NM_004235 | |
Other Reprogramming Factors: | 34 | C10 | LIN28A | CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A | Lin-28 homolog A (C. elegans) | NM_024674 |
35 | C11 | NANOG | – | Nanog homeobox | NM_024865 | |
36 | C12 | NR5A2 | B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2 | Nuclear receptor subfamily 5, group A, member 2 | NM_003822 | |
Reprogramming Enhancers & Inhibitors | 37 | D1 | UTF1 | – | Undifferentiated embryonic cell transcription factor 1 | NM_003577 |
ESC Markers | 38 | D2 | ALPL | AP-TNAP, APTNAP, HOPS, TNAP, TNSALP | Alkaline phosphatase, liver/bone/kidney | NM_000478 |
39 | D3 | GDF3 | KFS3, MCOP7, MCOPCB6 | Growth differentiation factor 3 | NM_020634 | |
40 | D4 | TDGF1 (CRIPTO) | CR, CRGF, CRIPTO | Teratocarcinoma-derived growth factor 1 | NM_003212 | |
Other ESC-Related Genes: . | 41 | D5 | CCNA2 | CCN1, CCNA | Cyclin A2 | NM_001237 |
42 | D6 | CDK1 (CDC2) | CDC2, CDC28A, P34CDC2 | Cyclin-dependent kinase 1 | NM_001786 | |
43 | D7 | CDC42 | CDC42Hs, G25K | Cell division cycle 42 (GTP binding protein, 25kDa) | NM_001791 | |
44 | D8 | DPPA2 | CT100, ECAT15-2, PESCRG1 | Developmental pluripotency associated 2 | NM_138815 | |
45 | D9 | DPPA3 | STELLA | Developmental pluripotency associated 3 | NM_199286 | |
46 | D10 | FGF2 | BFGF, FGF-2, FGFB, HBGF-2 | Fibroblast growth factor 2 (basic) | NM_002006 | |
47 | D11 | HSPA9 | CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74 | Heat shock 70kDa protein 9 (mortalin) | NM_004134 | |
48 | D12 | MYBL2 | B-MYB, BMYB | V-myb myeloblastosis viral oncogene homolog (avian)-like 2 | NM_002466 | |
49 | E1 | OTX2 | CPHD6, MCOPS5 | Orthodenticle homeobox 2 | NM_021728 | |
50 | E2 | SOX15 | SOX20, SOX26, SOX27 | SRY (sex determining region Y)-box 15 | NM_006942 | |
51 | E3 | TBX3 | TBX3-ISO, UMS, XHL | T-box 3 | NM_016569 | |
52 | E4 | TCF3 | E2A, E47, ITF1, TCF-3, VDIR, bHLHb21 | Transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) | NM_003200 | |
Blood Stem Cell: | 53 | E5 | CD34 | – | CD34 molecule | NM_001773 |
Keratinocyte: | 54 | E6 | GJB2 | CX26, DFNA3, DFNA3A, DFNB1, DFNB1A, HID, KID, NSRD1, PPK | Gap junction protein, beta 2, 26kDa | NM_004004 |
55 | E7 | KRT15 | CK15, K15, K1CO | Keratin 15 | NM_002275 | |
Cartilage: | 56 | E8 | COL1A1 | OI4 | Collagen, type I, alpha 1 | NM_000088 |
57 | E9 | COL2A1 | ANFH, AOM, COL11A3, SEDC, STL1 | Collagen, type II, alpha 1 | NM_001844 | |
Neuronal Stem Cell: | 58 | E10 | EMX2 | – | Empty spiracles homeobox 2 | NM_004098 |
59 | E11 | FABP7 | B-FABP, BLBP, FABPB, MRG | Fatty acid binding protein 7, brain | NM_001446 | |
60 | E12 | NCAM1 | CD56, MSK39, NCAM | Neural cell adhesion molecule 1 | NM_000615 | |
61 | F1 | NES | – | Nestin | NM_006617 | |
62 | F2 | NUMB | C14orf41, S171, c14_5527 | Numb homolog (Drosophila) | NM_003744 | |
63 | F3 | OLIG2 | BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19 | Oligodendrocyte lineage transcription factor 2 | NM_005806 | |
Cardiomyocyte | 64 | F4 | PECAM1 | CD31, CD31, EndoCAM, GPIIA‘, PECA1, PECAM-1, endoCAM | Platelet/endothelial cell adhesion molecule | NM_000442 |
Germ Cells | 65 | F5 | SYCP3 | COR1, SCP3, SPGF4 | Synaptonemal complex protein 3 | NM_153694 |
Ectoderm: | 66 | F6 | PAX6 | AN, AN2, D11S812E, MGDA, WAGR | Paired box 6 | NM_000280 |
67 | F7 | TUBB3 | CDCBM, CDCBM1, CFEOM3A, TUBB4, beta-4 | Tubulin, beta 3 | NM_006086 | |
68 | F8 | FGF8 | AIGF, FGF-8, HBGF-8, HH6, KAL6 | fibroblast growth factor 8 (androgen-induced) | NM_006119 | |
69 | F9 | FN1 | CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF | fibronectin 1 | NM_212482.2 | |
70 | F10 | GFAP | glial fibrillary acidic protein | NM_002055 | ||
71 | F11 | NEUROD1 | BETA2, BHF-1, MODY6, NEUROD, bHLHa3 | neuronal differentiation 1 | NM_002500 | |
72 | F12 | TH | DYT14, DYT5b, TYH | tyrosine hydroxylase | NM_199293 | |
Mesoderm: | 73 | G1 | BMP4 | BMP2B, BMP2B1, MCOPS6, OFC11, ZYME | Bone morphogenetic protein 4 | NM_001202 |
74 | G2 | GATA2 | DCML, MONOMAC, NFE1B | GATA binding protein 2 | NM_032638 | |
75 | G3 | HAND1 | Hxt, Thing1, bHLHa27, eHand | Heart and neural crest derivatives expressed 1 | NM_004821 | |
76 | G4 | MESP1 | bHLHc5 | Mesoderm posterior 1 homolog (mouse) | NM_018670 | |
77 | G5 | HBB | CD113t-C, beta-globin | hemoglobin, beta | NM_000518 | |
78 | G6 | RUNX1 | AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB | Runt-related transcription factor 1 | NM_001754 | |
Endoderm: | 79 | G7 | FOXA2 | HNF3B, TCF3B | Forkhead box A2 | NM_021784 |
80 | G8 | GATA4 | ASD2, TACHD, VSD1 | GATA binding protein 4 | NM_002052 | |
81 | G9 | HHEX | HEX, HMPH, HOX11L-PEN, PRH, PRHX | Hematopoietically Expressed Homeobox | NM_002729 | |
82 | G10 | HNF4A | HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14 | Hepatocyte nuclear factor 4, alpha | NM_178849 | |
83 | G11 | SOX17 | VUR3 | SRY (sex determining region Y)-box 17 | NM_022454 | |
84 | G12 | EOMES | TBR2 | eomesodermin | NM_005442.3 | |
Naive gene markers | 85 | H1 | KLF2 | LKLF | Kruppel-like factor 2 (lung) | NM_016270.3 |
86 | H2 | TFCP2L1 | CRTR1, LBP-9, LBP9 | Homo sapiens transcription factor CP2-like 1 (TFCP2L1) | NM_014553.2 | |
87 | H3 | DNMT1 | ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT | DNA (cytosine-5-)-methyltransferase 1 | NM_001130823.2 NM_001379.3 |
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88 | H4 | DNMT3A | DNMT3A2, M.HsaIIIA | DNA (cytosine-5-)-methyltransferase 3 alpha | NM_175629.2 NM_153759.3 NM_022552.4 |
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89 | H5 | DNMT3L | DNA (cytosine-5-)-methyltransferase 3-like | NM_013369.3 NM_175867.2 |
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90 | H6 | XIST | DXS1089, DXS399E, LINC00001, NCRNA00001, SXI1, swd66 | X inactive specific transcript (non-protein coding) | NR_001564.2 | |
House keeping genes | 91 | H7 | GAPDH | G3PD, GAPD, HEL-S-162eP | glyceraldehyde-3-phosphate dehydrogenase | NM_002046.6 |
92 | H8 | B2M | IMD43 | beta-2-microglobulin | NM_004048.3 | |
93 | H9 | SDHA | CMD1GG, FP, PGL5, SDH1, SDH2, SDHF | succinate dehydrogenase complex flavoprotein subunit A | NM_004168.3 | |
94 | H10 | HPRT1 | HGPRT, HPRT | hypoxanthine phosphoribosyltransferase 1 | NM_000194.2 | |
NO-RT control (primery GAPDH) | 95 | H11 | NO-RT control | |||
PCR positive control (primery ACTB) | 96 | H12 | PCR positive control |