Integrity of DNA/RNA is very important test needed according to the Europe pharmacopeia chapter 5.14.
Mostly the test can be done as agarose gel electrophoresis or using advanced techniques such as PCR or fragment analysis.
All the tests are according to the Europe pharmacopeia test 2.6.21.
To this test belong also plasmid DNA conformation test, because Stability of plasmid DNA depends on its conformation and the right conformation is required (e.g. supercoiled form).
For RNA integrity determination of RIN is important as well.
gb SG PCR Master Mix gb SG PCR Master Mix je určen pro real-time PCR analýzu, kde sybrgreen (dále jen SG) nahrazuje použití fluorescenčně značené sondy. Master Mix PCR lze využít také v end-point PCR analýze. Jeho výhodou je zvýšená odolnost proti PCR inhibitorům.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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