Although food and feed testing does not fall into pharma testing, some QC tests are based on similar techniques and methods as those used in Good Manufacturing Practice.
European Food Safety Authority (EFSA) requires many QC processes before food or feed is released to the market. Some of them are based on DNA/RNA testing and they are very similar to those we provide in the scope of pharma testing, such as: DNA/RNA based detection of biological hazards, detection of DNA contaminants in feed additives, detection of genetically modified (GM) food or feed.
There are two main areas where GENERI BIOTECH can provide an impact:
The first area is the testing itself, especially when a method used is complicated and may not be performed efficiently on a routine basis.
The other area is the development of methods and tools for effective routine testing in manufacturer´s laboratory.
Duálně značené hydrolyzační sondy Technologie duálně značených hydrolyzačních sond (tzv. TaqMan® sondy) využívá 5 ́-3 ́ exonukleázové aktivity Taq DNA polymerázy ke štěpení duálně značené hydrolyzační sondy. Hydrolyzační sondy jsou značeny na 5´ konci fluorescenčním barvivem (reporterem) a na 3´ konci zhášečem (quencherem).
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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