Mycoplasma contaminants may be introduced into cell culture and other biological products through master seeds, master cell seed (stock), starting materials of animal origin, and in processing of biological materials during passage and product assembly. It is therefore necessary to demonstrate through testing that Mycoplasmas are not present in the final product, working seeds and cells and harvests, and starting materials such as master seed, master cell seed, and ingredients of animal origin.
The test is done in compliance with Europe pharmacopeia test 2.6.7.
Duálně značené hydrolyzační sondy Technologie duálně značených hydrolyzačních sond (tzv. TaqMan® sondy) využívá 5 ́-3 ́ exonukleázové aktivity Taq DNA polymerázy ke štěpení duálně značené hydrolyzační sondy. Hydrolyzační sondy jsou značeny na 5´ konci fluorescenčním barvivem (reporterem) a na 3´ konci zhášečem (quencherem).
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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