Cell and gene therapy are new branches of pharmaceutical research, which use new types of drugs and strategies. New types of analytical methods are obviously needed. These methods are mostly based on DNA or RNA analysis, which is not surprising, as nucleic acid such as viral vectors, naked DNA (like plasmid, oligonucleotide) or RNA, as active agens is typically used for methods of gene therapy.
If you want to measure distribution or degradation of your DNA drug, molecular biology methods are the first choice. These methods are based on PCR and are very sensitive and selective. Only few copies of DNA in mixture of host organism DNA should be detected. And looking for a needle in a haystack is exactly the work for GENERI BIOTECH, who is regarded an expert in DNA diagnostic and analysis for more than 20 years.
GENERI BIOTECH specializes in analysis of nucleic acids, especially those needed in biodistribution and safety studies for gene therapy drugs. We have experiences with different types of tissues/matrices analysis, where we take into account special needs of each matrix type – isolation, inhibitors, etc.
Method development and validation, that is tailored to fit our customers‘ needs and meets requirements of the authorities, is a matter of course to us. Sample analysis, handling and reporting is an integral part of a study, as well as consulting a study plan or auditing by the customer.
Typical target material
Duálně značené hydrolyzační sondy Technologie duálně značených hydrolyzačních sond (tzv. TaqMan® sondy) využívá 5 ́-3 ́ exonukleázové aktivity Taq DNA polymerázy ke štěpení duálně značené hydrolyzační sondy. Hydrolyzační sondy jsou značeny na 5´ konci fluorescenčním barvivem (reporterem) a na 3´ konci zhášečem (quencherem).
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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