OLIGO & PROBES

We are EN ISO 9001, 13485 and 17025, respectively, certified EU-located company manufacturing olignucleotides on cutomers´ demand. We have been synthesizing oligonucleotides from 1995. Every year we produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications, for various purposes (biological techniques mostly based on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

We implemented the know-how we acquired in the field of oligo synthesis during last 23 years to the development of other products, especially IVD kits and Life Sciences products.

Our typical partners are end-users of the oligonucleotides (customers mostly in CZ and SK), business partners and distributors mostly within EU. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, to their sales strategy.

Custom oligo synthesis

Custom oligo synthesis Custom oligo synthesis

Oligonucleotides are synthesized in GENERI BIOTECH on Highly cross-linked polystyrene beads support. Oligonucleotides thus synthesized have a very low representation of unsynctified shorter chains compared to normal CPG support.

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GEMINI™ probes

GEMINI™ probes GEMINI™ probes

GEMINI™ probes are a type of real-time PCR probe developed in GENERI BIOTECH. The technology of GEMINI is based on placing internal quenchers inside the probe sequence that results in a stronger quenching and a lower background signal. The probes are HPLC purified (no additional fee).

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SEQplus sequencing primers

SEQplus sequencing primers SEQplus sequencing primers

5´-SEQplus modification at the sequencing primer enables reading of sequence from the 2nd nucleotide of the sequenced DNA. We offer this modification for any user´s sequence or as ready-to-use sequencing primers in stock.

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Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave dual-labeled hydrolysis probes. Hydrolysis probes are labeled at the 5´ end with a fluorescent dye (reporter) and at the 3´ end with a quencher.

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Single-labeled hybridization probes – FRET probes

Single-labeled hybridization probes – FRET probes Single-labeled hybridization probes – FRET probes

The system of single-labeled probes (also called FRET probes - Fluorescence Resonance Energy Transfer) consists of a pair of probes, the former is labeled with donor fluorophore (3´FAM) at the 3 ́ end, the latter is labeled with acceptor fluorophore (5´CFR 610 or 635) at the 5 ́ end. The probes are designed to hybridize PCR targets adjacent to each other. The probes are HPLC purified (no additional fee).

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NGS Primers

NGS Primers NGS Primers

Primers for NGS platforms (Next Generation Sequencing) to HPLC quality are available at a scale of 20 nmol.

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oligo DUPLEX

oligo DUPLEX oligo DUPLEX

We can prepare a double-chain DNA (oligo DUPLEX) from complementary single-chain oligonucleotides (ssDNA). Duplex can be prepared from oligonucleotides ordered by you, or we can design complementary ssDNA to oligo supplied by you.

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Inosin

Inosin Inosin

2'deoxyinosin is a "universal" base (it has the ability to bind to each of the 4 standard bases). It can be located anywhere in the oligonucleotide chain.

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