OLIGO & PROBES

We are EN ISO 9001, 13485 and 17025, respectively, certified EU-located company manufacturing olignucleotides on cutomers´ demand. We have been synthesizing oligonucleotides from 1995. Every year we produce dozens of thousands of oligos in many categories: unmodified oligos, variously modified oligos, oligos with various types of purifications, for various purposes (biological techniques mostly based on PCR, NGS, biophysical studies etc.). We produce real-time PCR probes as well.

We implemented the know-how we acquired in the field of oligo synthesis during last 23 years to the development of other products, especially IVD kits and Life Sciences products.

Our typical partners are end-users of the oligonucleotides (customers mostly in CZ and SK), business partners and distributors mostly within EU. We are looking for business partners and/or distributors worldwide who can fit the portfolio of our products, either the whole or a part of it, to their sales strategy.

2’F-RNA

2’F-RNA 2’F-RNA

2'-F-RNA is even more stable than 2'-O-methyl-RNA. 2'-F-RNA & RNA duplexes do not activate RNase H and are more stable (with higher Tm) than RNA & 2'-O-methyl-RNA duplexes. It serves mainly for antisense applications. May be attached anywhere within the oligonucleotide sequence.

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2’OMe-RNA

2’OMe-RNA 2’OMe-RNA

Compared to RNA 2'-O-methyl-RNA is more chemically stable (being stable against nucleases for several days). 2'-O-methyl-RNA & RNA duplexes do not activate RNase H and are more stable (with higher Tm) than DNA & RNA duplexes. It is used mainly in antisense applications. May be attached anywhere within the oligonucleotide sequence.

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2’3′-ddC

2’3′-ddC 2’3′-ddC

It serves as a blocker of 3' polymerase extension. 2',3'-ddC may be attached to the 3' end of the oligonucleotide.

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3-Deaza-dA

3-Deaza-dA 3-Deaza-dA

It is used to study oligonucleotide activity changes following alteration of the main structural component. 3-Deaza-dA may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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5-(1-Propynyl)-dC (pdC)

5-(1-Propynyl)-dC (pdC) 5-(1-Propynyl)-dC (pdC)

It is used to increase duplex stability and melting temperature while preserving specificity of hybridization. 5-(1-Propynyl)-dC may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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5-(1-Propynyl)-dU (pdU)

5-(1-Propynyl)-dU (pdU) 5-(1-Propynyl)-dU (pdU)

It is used to increase duplex stability and melting temperature while preserving specificity of hybridization. 5-(1-Propynyl)-dU may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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Biotin-TEG

Biotin-TEG Biotin-TEG

Biotin is ligated to the oligonucleotide by means of a triethylene glycol spacer (TEG), extending the distance between the oligonucleotide and the biotin by 15 atoms. Biotin-TEG may be ligated to the 3 'end of the oligonucleotide.

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ROX

ROX ROX

ROX (6-Carboxy-X-Rhodamine) is a red fluorophore. It can be linked to the 3 'end of the chain, or by using the NHS ester at 5'. In case of binding at the 5 'end, the amino linker is first attached to the oligonucleotide through which the post-synthetic binding of ROX then proceeds.

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Spermine

Spermine Spermine

It is used for manufacturing of oligospermine-oligonucleotide conjugates (Zip Nucleic Acid - ZNA Oligos). ZNA oligos are employed e.g. in multiplex PCR, PCR of AT-rich sequences, RT qPCR, miRNA detection and improving SNP discrimination. Spermine may be attached anywhere within the oligonucleotide sequence except for the 3' end.

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