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ngs ONCO anti-EGFR practical WORKSHOP

Date will be specified.

Organized by: GENERI BIOTECH with cooperation of VUAB Pharma
Location: VUAB Pharma R&D laboratories, Vltavská 53, Roztoky CZ-25263

 

Day 1

9:00 – 9:10 Introduction
9:10 – 10:00 Next-generation sequencing essentials
Sequencing-by-synthesis technology
Use of the NGS in diagnostics
10:00 – 10:45 Presentation of the ngs ONCO anti-EGFR kit
Library preparation workflow
10:45 – 11:05 Coffee break
11:00 – 12:30 Hands-on part I
Input DNA sample QC step
Sample-sheet generation
1st PCR amplification
12:30 – 13:30 Lunch
13:30 – 16:00 Hands-on part II
Sample indexing – 2nd PCR amplification
Magnetic beads purification step
QC steps in library preparation (Qubit, 2100 Bioanalyzer)

Day 2

9:00 – 10:45 Hands-on part III
Sample pooling and library denaturation
Sequencing on the Illumina MiSeq
10:45 – 11:00 Coffee break
11:00 – 12:30 Sequencing data evaluation
Introduction of the ERMI evaluation software
Practical work with sequencing data
12:30 – 13:30 Lunch
13:30 – 14:00 Discussion

 

The workshop will be held in English.

Registration