Outputs of custom oligo synthesis

Aliquoted oligos

The yield of oligo is divided either into a  pre-defined number of microtubes or according to pre-defined amount of oligo per each vial.

Output: an identical oligo divided into several microtubes (one oligo – several vials)

Benefit: easier handling, decreased risk of contamination

Oligos dissolved to 100 μM concentration

The whole yield of oligo is dissolved in redistilled water to a concentration of 100 μM.

Output: each oligo as a 100 μM solution in a microtube (one oligo – one vial)

Benefit: easier handling of a high number of oligos 

Oligos on deep 96-well plate

Oligos are put into a deep 96-well plate according to customer´s requirements (one well – one oligo)

Output: oligos on array are normally delivered dried in 2-ML wells of an array.  Due to 2-ML volume of the well customers can dissolve oligos using standard methods.

Customers can select delivery on a preferred type of array, delivery of either dried oligos or oligos in solution, number of arrays with aliquots.

Oligos in the deep 96-well plate can be:

• unadjusted / dried (A) – the whole yield of oligo is put into the well – thus each well may contain different amounts of oligo
• adjusted to one equimolar value / dried (B) – all the wells contain the same molar amount of oligo – thus to make the same concentration of oligos, the same volume of deionized water is added to all the wells
• adjusted to one equimolar value / dissolved to 100 μM solution (C) – each well contains the same volume of 100 μM oligo solution

Benefit: oligos on array are recommended for instrumental applications requiring high number of oligos, such as anchoring oligos to microarrays, or other automated methods where handling oligos delivered in vials may be ineffective.

Oligos on 96-well microplate

Oligos are put into a 96-well microplate according to customer´s requirements (typically: one well – one oligo). The plate is typically inteded for PCR protocols.

Output: oligos on array are normally delivered dried in wells of a microplate.

Oligos in the 96-well microplates are usually:

• adjusted to one equimolar value / dried (D) – each well contains the same amount of oligo – all the oligos on the array can thus be dissolved with the same volume of water/buffer

Benefit: oligos on array are recommended for instrumental applications requiring high number of oligos, such as anchoring oligos to microarrays, or other automated methods where handling oligos delivered in vials may be ineffective.

When the whole yield of a set of oligos is aliquoted to many microplates, one microplate is usualy consumpted for one test/experiment, whereas the others are untouched, ready for furhther tests/experiments.