MALDI-TOF spectrometers is routinely used to check the quality of our oligonucleotides.
MALDI-TOF mass spectrometry
MALDI-TOF (Matrix Assisted Laser Desorption/Ionization – Time Of Flight) is an analytical method based on laser ionisation of the analyte (oligonucleotide) molecule with the support of the matrix material. Excitation is followed by determining the time needed by the ionised molecule to “fly” along an evacuated tube towards a detector. Mass spectrum analysis allows very accurate comparison of predicted and real molecular mass and also recognition of respective impurities. This enables highly reliable quality assessment of a synthetized sequence.
MALDI-TOF analysis is routinely performed in a representative sample of oligonucleotides from every synthesis run and also in all oligonucleotides at risk of lower quality (e. g. in modified oligos). The customer is not informed of these analyses.