What OLIGO generator does
Here you can generate multiple random DNA/RNA sequences with specified percentages of
individual bases.
The sequences can be exported to file or coppied to clipboard to be subsequently pasted
into an excel spreadsheet.
Generated sequences
What OLIGO converter does
Here you can convert multiple DNA/RNA sequences simultaneously into their reverse,
complement and
reverse-complement counterparts.
The resulting sequences can be exported to file or copied to clipboard to be
subsequently
pasted
into an excel spreadsheet.
How to do it
Restrictions
Useful hints
total sequences: format detected: |
---|
Converted sequences
Select | No. | Type | Name | Sequence | Analyse |
---|
What OLIGO analyzer does
Here you can calculate basic characteristics of your oligonucleotide including GC content,
MW and OD, among others.
You can also observe the relations between the amount of substance, weight, OD and copy
number
values of your oligo.
Check:
Basic characteristics | |
---|---|
Length | - |
Count | - |
AT/U (%) | - |
GC (%) | - |
I (%) | - |
MW | - |
OD (1 µmol) | - |
Tm (°C) | - |
What OLIGO worker does
Activate the desired section by clicking on the relevant button – Dissolving / Dilution /Amounts. This tool helps you perform the frequently needed calculations when working with oligos in a lab, which are dissolving, dilutions and calculating the total amounts of oligos needed for particular experiments.
How to do it
Useful hints
Oligo type | Purification | Scale / amount | Guaranteed yield | Usual yield |
---|---|---|---|---|
Oligo |
|
|
- | - |
Probe | always HPLC |
|
- | - |