gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
- 5-(1-Propynyl)-dC (pdC)
- 5-(1-Propynyl)-dU (pdU)
- Amino C3sp
- Amino C6sp
- Amino C6sp-dA
- Amino C6sp-dC
- Amino C6sp-dT
- Amino C7sp
- Amino N2-C6sp-dG
- Biotin dT
- CFR 610
- CFR 635
- Spacer 9
- Spacer 18
- Spacer C3
- Spacer C12
- Symetric doubler
- Thiol SH
- Thiol S-S
- Yakima Yellow
- ATTO dyes
- sequencing primers
- real-time PCR probes
Modification by group: Modified bases
Position of modification: 5´, internal
It is used to increase duplex stability and melting temperature while preserving specificity of hybridization. 5-(1-Propynyl)-dC may be attached anywhere within the oligonucleotide sequence except for the 3′ end.
|1175||5-(1-Propynyl)-dC (pdC)||40 nmol||
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|1175||5-(1-Propynyl)-dC (pdC)||200 nmol||
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Modification is limited and can be performed upon consultation; precise price quotation can be prepared after providing particular sequences.
Oligo price = price of sequence without modification + surcharge for modification
Shiping at ambient temperature from
€ 35 (EU)
For other destinations see indicative list.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
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