Categories

Adenovirus detection

Adenovirus detection

Adenoviral vectors serve as oncolytic viruses in many applications and our laboratory has experiences with detection method development, validation and sample analysis.

Good example for adenoviral vectors is CGTG-102 (or ONCOS-102), which is in clinical phase now.

While the CGTG-102 oncolytic adenovirus has shown efficacy as a single agent against several soft tissue sarcomas, it would also be appealing to use in combination with other regimes, as oncolytic viruses have demonstrated very little overlap in side effects with traditional therapies such as chemotherapy and radiation. CGTG-102 has recently been studied in combination with doxorubicin, and a synergistic effect was observed. At least part of doxorubicin’s mechanism of action is as an inducer of immunogenic cell death, and it has been suggested that immune response contributes to its overall anti-tumor activity. Doxorubicin has been shown to increase adenoviral replication in soft tissue sarcoma cells as well, potentially contributing to the observed synergistic effect in the virus/doxorubicin combination.

While in phase I was already used to treat 200 advanced cancer patients in the company’s Advanced Therapy Access Program.

Source: Hemminki, Akseli (2014). “Oncolytic Immunotherapy: Where Are We Clinically?”. Scientifica. 2014: 862925. doi:10.1155/2014/862925. PMC 3914551. PMID 24551478.

Useful links
Recommended products
gb SG PCR Master Mix

gb SG PCR Master Mix gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

More