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General Terms and Conditions
Authentication of cell lines
Using a forensic kit, selected autosomal polymorphisms are amplified in a multiplex PCR. The polymorphic fragments of DNA are then separated in capillary electrophoresis. The same method is applied as well in forensic testing.
Purpose of the service
In the tested cell line, a profile of genomic DNA is carried out using fragmentation analysis. The profile is then compared to a previously published reference profile. After comparison of the two profiles, it is revealed if the profile of the tested cell line is identical with the reference profile or if it contains contamination from a different cell line.
In research experiments in life sciences, using a misidentified or cross-contaminated cell line concludes in faulty experimental data and unreproducible results. Cell phenotype often changes when the cell passage number is too high. It is estimated that 18 – 36 % of all cell lines used for research experiments were misidentified or crosscontaminated by a different cell line. Some scientific journals recommend that cell lines are authenticated prior to publishing an article. 1 J.R. Masters, J.A. Thomson, B. Daly-Burns, Y.A. Reid, W.G. Dirks, P. Packer, L.H. Toji, T. Ohno, H. Tanabe, C.F. Arlett, L.R. Kelland, M. Harrison, A. Virmani, T.H. Ward, K.L. Ayres and P.G. Debenham: Short tandem repeat profiling provides an international reference standard for human cell lines; PNAS 2001; 98(14): 8012-8017; doi:10.1073/pnas.121616198
Parameters of the test
The test can comprise:
- comparison of the cell line to the reference profile
- comparison of two cell lines (e.g. differing in cell passage number)
- detection of contamination by a different cell line
Recommendation when cell lines should be tested
- prior to the start of a long-term experiment and after it finishes
- when a contamination is suspected
- prior to storage of a cell line
Requirements for a material to be tested
Customer provides us either with an extracted genomic DNA or a yielded cell pellet.
Sample delivery
If the delivery time does not exceed 24 hours, send samples chilled to 2 to 8 °C (on wet ice), otherwise we recommend delivery on dry ice.
Output of the test
We provide you with an protocol in English.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 1860 | Authentication of cell line - human |
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| 1861 | Authentication of cell line - human, DNA extraction |
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Shiping at ambient temperature from
€ 40 (EU)
For other destinations see
indicative list.
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Human Cell Line Authentication on LabCard
LabCards are intended for simple transport of a cell sample or genomic DNA of human cell lines. Samples applied to the LabCard do not need to be transported chilled. STR analysis is performed as part of the service.
PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.