In the tested cell line, a profile of genomic DNA is carried out using fragmentation analysis. The profile is then compared to a previously published reference profile. After comparison of the two profiles, it is revealed if the profile of the tested cell line is identical with the reference profile or if it contains contamination from a different cell line.
In research experiments in life sciences, using a misidentified or cross-contaminated cell line concludes in faulty experimental data and unreproducible results. Cell phenotype often changes when the cell passage number is too high. It is estimated that 18 – 36 % of all cell lines used for research experiments were misidentified or crosscontaminated by a different cell line. Some scientific journals recommend that cell lines are authenticated prior to publishing an article. 1 J.R. Masters, J.A. Thomson, B. Daly-Burns, Y.A. Reid, W.G. Dirks, P. Packer, L.H. Toji, T. Ohno, H. Tanabe, C.F. Arlett, L.R. Kelland, M. Harrison, A. Virmani, T.H. Ward, K.L. Ayres and P.G. Debenham: Short tandem repeat profiling provides an international reference standard for human cell lines; PNAS 2001; 98(14): 8012-8017; doi:10.1073/pnas.121616198
Parameters of the test
The test can comprise:
- comparison of the cell line to the reference profile
- comparison of two cell lines (e.g. differing in cell passage number)
- detection of contamination by a different cell line
Recommendation when cell lines should be tested
- prior to the start of a long-term experiment and after it finishes
- when a contamination is suspected
- prior to storage of a cell line
Requirements for a material to be tested
Customer provides us either with an extracted genomic DNA or a yielded cell pellet.
If the delivery time does not exceed 24 hours, send samples chilled to 2 to 8 °C (on wet ice), otherwise we recommend delivery on dry ice.
Output of the test
We provide you with an accredited protocol in English.