Using a forensic kit, selected autosomal polymorphisms are amplified in a multiplex PCR. The polymorphic fragments of DNA are then separated in capillary electrophoresis. The same method is applied as well in forensic testing.
In the tested cell line, a profile of genomic DNA is carried out using fragmentation analysis. The profile is then compared to a previously published reference profile. After comparison of the two profiles, it is revealed if the profile of the tested cell line is identical with the reference profile or if it contains contamination from a different cell line.
In research experiments in life sciences, using a misidentified or cross-contaminated cell line concludes in faulty experimental data and unreproducible results. Cell phenotype often changes when the cell passage number is too high. It is estimated that 18 – 36 % of all cell lines used for research experiments were misidentified or crosscontaminated by a different cell line. Some scientific journals recommend that cell lines are authenticated prior to publishing an article. 1 J.R. Masters, J.A. Thomson, B. Daly-Burns, Y.A. Reid, W.G. Dirks, P. Packer, L.H. Toji, T. Ohno, H. Tanabe, C.F. Arlett, L.R. Kelland, M. Harrison, A. Virmani, T.H. Ward, K.L. Ayres and P.G. Debenham: Short tandem repeat profiling provides an international reference standard for human cell lines; PNAS 2001; 98(14): 8012-8017; doi:10.1073/pnas.121616198
The test can comprise:
Customer provides us either with an extracted genomic DNA or a yielded cell pellet.
If the delivery time does not exceed 24 hours, send samples chilled to 2 to 8 °C (on wet ice), otherwise we recommend delivery on dry ice.
We provide you with an protocol in English.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 1860 | Authentication of cell line - human |
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| 1861 | Authentication of cell line - human, DNA extraction |
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Shiping at ambient temperature from
€ 37 (EU)
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indicative list.
General Terms and Conditions
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional
The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
Biodistribution (BD) studies are critical components when assessing the preclinical safety evaluation of cell and gene therapy molecules. BD analysis is generally conducted at the molecular level using a methodology that provides high sensitivity.
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