Categories

Authentication of cell lines

Authentication of cell lines

Using a forensic kit, selected autosomal polymorphisms are amplified in a multiplex PCR. The polymorphic fragments of DNA are then separated in capillary electrophoresis. The same method is applied as well in forensic testing.

Purpose of the service

In the tested cell line, a profile of genomic DNA is carried out using fragmentation analysis. The profile is then compared to a previously published reference profile. After comparison of the two profiles, it is revealed if the profile of the tested cell line is identical with the reference profile or if it contains contamination from a different cell line.

In research experiments in life sciences, using a misidentified or cross-contaminated cell line concludes in faulty experimental data and unreproducible results. Cell phenotype often changes when the cell passage number is too high. It is estimated that 18 – 36 % of all cell lines used for research experiments were misidentified or crosscontaminated by a different cell line. Some scientific journals recommend that cell lines are authenticated prior to publishing an article. 1 J.R. Masters, J.A. Thomson, B. Daly-Burns, Y.A. Reid, W.G. Dirks, P. Packer, L.H. Toji, T. Ohno, H. Tanabe, C.F. Arlett, L.R. Kelland, M. Harrison, A. Virmani, T.H. Ward, K.L. Ayres and P.G. Debenham: Short tandem repeat profiling provides an international reference standard for human cell lines; PNAS 2001; 98(14): 8012-8017; doi:10.1073/pnas.121616198

Parameters of the test

The test can comprise:

  • comparison of the cell line to the reference profile
  • comparison of two cell lines (e.g. differing in cell passage number)
  • detection of contamination by a different cell line

Recommendation when cell lines should be tested

  • prior to the start of a long-term experiment and after it finishes
  • when a contamination is suspected
  • prior to storage of a cell line

Requirements for a material to be tested

Customer provides us either with an extracted genomic DNA or a yielded cell pellet.

Sample delivery

If the delivery time does not exceed 24 hours, send samples chilled to 2 to 8 °C (on wet ice), otherwise we recommend delivery on dry ice.

Output of the test

We provide you with an protocol in English.

Cat. No. Product No. of reactions Price
1860 Authentication of cell line - human
Add to Quote
Add to Quote
1861 Authentication of cell line - human, DNA extraction
Add to Quote
Add to Quote

Shiping at ambient temperature from € 37 (EU)
For other destinations see indicative list.

General Terms and Conditions

Useful links
Recommended products
Dual-labeled hydrolysis probes

Dual-labeled hydrolysis probes Dual-labeled hydrolysis probes

The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional

More