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gb SARS-CoV-2 Combi gb SARS-CoV-2 Combi

gb SARS-CoV-2 Combi

Detection method: one-step real-time PCR

Combi CE IVD kit for detection of SARS-CoV-2 virus is based on one-step RT-qPCR using fluorescently labelled probes which allow the detection of viral genes E and RdRP in FAM channel and external positive control in HEX channel. The detection method is based on officially recommended WHO and Institute Pasteur designs for detection of SARS-Cov-2 and usage of our proprietary GEMINI™ probe technology.

Product description

– CE IVD one-step RT-qPCR kit
– combi assay targeting the viral E gene and RdRP genes in FAM channel and the External Positive Control in HEX channel
– the kit includes Master Mix OneStep Multi, Positive Control CoV-2, EPC Template RNA and Deionized Water
– Positive Control CoV-2 serves for a verification of the analysis validity
– EPC Template RNA is an external positive control for verification of the isolation process
– GEMINI™ probe technology results in high sensitivity of detection and lower background
LOD of combi assay is 3,45 copies of viral RNA per reaction (95% CI)
– the kit contains all the necessary components to perform the test
suitable for two channel PCR thermocyclers
– identical temperature profile for all SARS-CoV-2 detection kits
– analysis preparation scheme

Prevention of false negative results

In the kit, control of both RNA extraction step and PCR process is provided by detection of external RNA in HEX channel after spiking the samples with the external RNA prior to the extraction.
In addition to this generally used protocol, the labs that demand a verification of the sampling step can use our gb Human B2M mRNA kit. The kit is based on the detection of transcripts of B2M gene, a human reference gene ubiquitously present in all human samples. If the human reference transcript is not detected, the sample was not taken properly.

 

Cat. No. Product No. of reactions Price
3232-100 gb SARS-CoV-2 Combi 100
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3232-500 gb SARS-CoV-2 Combi 500
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CE IVD one-step RT-qPCR kit contains reagents to provide 100 or 500 reactions (20 μl volume of each reaction).

Shiping on dry ice from € 95 (EU)
For other destinations see indicative list.

Validated for:

CFX96/96Touch (Bio-Rad)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

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