Customized High-Throughput PCR Array
These custom designed arrays contain oligos (primers and probes) for PCR or real-time PCR according to customer´s requirements.
Principle of the product
Preparation of oligos on array (mostly 96-well plate) is recommended when high number of PCR / real-time PCR tests are needed to be performed paralelly on one microplate. Typical applications are high-throughput molecular diagnotics or gene expression assays. After adding MasterMix + sample DNA, the plates are ready for PCR / real-time PCR. The main benefit of this service is making time-consuming laboratory protocols simpler and faster.
Product patameters
Possible layouts of the array for PCR methods:
- each well on plate contains the same pair of oligos (the plate is used for testing many samples paralelly)
- each well on plate contains different pair of oligos (the plate is used for many tests of one sample paralelly)
- combination of both
Possible layouts of the array for real-time PCR methods (those using PCR probe):
- each well on plate contains the same pair of oligos + real-time PCR probe (the plate is used for testing many samples paralelly)
- each well on plate contains different pair of oligos + real-time PCR probe (the plate is used for many tests of one sample paralelly)
- combination of both
Requirements for input data by customer
- design of layout of the array
- sequences of primers/probe
- quantity of primers/probe per well
- preferred type of array
- number of arrays to be manufactured
Output
- selected numbers of sealed arrays containing mix of primers /primers+probe in each well