gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
gb GENETIC APOB
Detection method: allelic discrimination
This in vitro diagnostic kit is intended for detection of mutation R3500Q in the gene APOB in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Clinical implications of the CE IVD kit
Apolipoprotein B100 (APOB100) is one of the B forms of apolipoprotein and it forms the basic protein component of lipoproteins with low and very low density (LDL, VLDL). Thus it plays a significant role during lipid and cholesterol transport. It is synthesized in the liver and distributed by the blood stream to the cells on whose surface it works as a ligand for LDL receptors mediating cholesterol transport to the cells.
The most common APOB gene mutation is an R3500Q mutation, which leads to the exchange of amino acid arginine (Arg) for glutamin (Gln) in position 3527. In such a way it causes a change in the protein structure in the place of the receptor binding. Consequently, there is a lower affinity of LDL particles towards the receptors and their accumulation in blood. This genetic disorder is called a familial defective Apolipoprotein B100 and it is one of the causes of familial hypercholesterolemia. Cholesterol accumulation in blood increases the risk of atherosclerosis and heart attack. Although the frequency of the mutant allele is only 0.1% of the healthy population, the frequency is up to 10% in individuals with familial hypercholesterolemia.
Parameters of the real-time PCR diagnostic kit
- ready-to-use assay
- sample concentration 1-100 ng/µl
- positive and negative controls included
- FAM and HEX channels detection
- identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
|Cat. No.||Product||No. of reactions||Price|
|3205-025||gb GENETIC APOB||25||
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1 CE IVD real-time PCR kit contains reagents to provide 25 PCR reactions (20 μl volume of each reaction).
Shiping on dry ice from
€ 100 (EU)
For other destinations see indicative list.
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