Detection method: melting analysis
This in vitro diagnostic kit is intended for detection of polymorphism TA (allele 7TA) in gene UGT1A1 in human genomic DNA. Detection is based on polymerase chain reaction (PCR) with analysis of melting curves using fluorescently labelled probe.
The gb GENETIC Gilbert diagnostic kit is used to detect changes in the number of TA repetitions in the promoter of the UGT1A1 gene. This gene encodes the enzyme UDPglucuronosyltransferase, which is responsible for the conjugation phase of biotransformation of bilirubin.
The unmutated gene variant contains 6 TA repeats in the promoter, the mutated variant most frequently 7 TA repeats. Detection of this mutation is useful before starting treatment with medications whose limited biotransformation can result in development of toxic effects. Genotyping enables timely dose reduction or selection of alternative therapy.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3216-025 | gb GENETIC Gilbert | 25 |
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| 3216-050 | gb GENETIC Gilbert | 50 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
ABI 7500/7500 Fast (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
Stratagene Mx3000P/Mx30005P (Agilent Technologies)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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