Detection method: real-time PCR
The kit is intended for detection of HLA-B27 allele in samples of human DNA. Detection principle is based on real-time PCR method with allele specific primers and fluorescently labeled hydrolyzation probes.
The presence of B27 antigen (HLA-B27) is strongly associated with ankylosing spondylitis (AS) and other inflammatory disorders. The frequency of HLA-B*27 allele in Caucasian population is in range 5–10% and in AS-patients occurs in more than 85% cases. The absence of HLA-B27 allele is a strong negative predictor for diagnosis of AS.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3257-025 | gb GENETIC HLA-B*27 | 25 |
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| 3257-100 | gb GENETIC HLA-B*27 | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction)
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About product
General Terms and Conditions
Validated for:
CFX96/96Touch (Bio-Rad)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
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The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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