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CZE General Terms and Conditions
gb GENETIC HLA-B*27
Detection method: real-time PCR
The kit is intended for detection of HLA-B27 allele in samples of human DNA. Detection principle is based on real-time PCR method with allele specific primers and fluorescently labeled hydrolyzation probes.
Clinical implications
The presence of B27 antigen (HLA-B27) is strongly associated with ankylosing spondylitis (AS) and other inflammatory disorders. The frequency of HLA-B*27 allele in Caucasian population is in range 5–10% and in AS-patients occurs in more than 85% cases. The absence of HLA-B27 allele is a strong negative predictor for diagnosis of AS.
Parameters of the diagnostic kit
- in vitro diagnostics
- CE IVD marked
- ready-to-use assay
- sample concentration 10-100 ng/µl
- positive and negative controls included
- FAM and HEX channels detection
- identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3257-025 | gb GENETIC HLA-B*27 | 25 |
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| 3257-100 | gb GENETIC HLA-B*27 | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction)
Shiping on dry ice from
€ 115 (EU)
For other destinations see
indicative list.
Validated for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
While HLA-B27 is by far the strongest and most well-known genetic marker linked with ankylosing spondylitis, several other genetic factors also contribute to disease susceptibility and can provide additional diagnostic or research insights. 1. HLA-B27 (primary marker) Present in ~90% of patients with AS of European ancestry. Not causative by itself — about 5–10% of
gb GENETIC FRUCTO
The CE IVD real-time PCR kit is intended for detection of mutation A149P, A174D, N334K and deletion del4E4 in the ALDOB gene in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
gb GENETIC HFE
The CE IVD real-time PCR kit is intended for detection of mutations C282Y, H63D and S65C in the gene HFE in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).