Detection method: real-time PCR

The kit is intended for detection of HLA-B27 allele in samples of human DNA. Detection principle is based on real-time PCR method with allele specific primers and fluorescently labeled hydrolyzation probes.

Clinical implications

The presence of B27 antigen (HLA-B27) is strongly associated with ankylosing spondylitis (AS) and other inflammatory disorders. The frequency of HLA-B*27 allele in Caucasian population is in range 5–10% and in AS-patients occurs in more than 85% cases. The absence of HLA-B27 allele is a strong negative predictor for diagnosis of AS.

Parameters of the diagnostic kit

  • in vitro diagnostics
  • CE IVD marked
  • ready-to-use assay
  • sample concentration 1-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3257-025 gb GENETIC HLA-B*27 25
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3257-100 gb GENETIC HLA-B*27 100
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0,4 ml equals to 25 PCR reactions of 20 μl

Shiping on dry ice from € 100 (EU)
For other destinations see indicative list.

Validated for:

CFX96/96Touch (Bio-Rad)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

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Useful links
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