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General Terms and Conditions
gb GENETIC HLA-B*27
Detection method: real-time PCR
The CE IVD real-time PCR kit intended for qualitative detection of B*27 alleles of B locus in the major histocompatibility complex (MHC) located on chromosome 6 encoding the Human leukocyte antigen B27 (HLA-B*27).
Parameters of the in vitro diagnostic kit
- CE IVD marked, IVDR certified in accordance with EU Regulation 2017/746
- ready-to-use assay
- sample concentration 10-100 ng/µl
- positive and negative controls included
- FAM and HEX channels detection
- identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
- detection is performed from human genomic DNA samples extracted from peripheral blood or buccal swabs
- kit requires manual preparation of reagents and samples which are then analysed on real-time PCR cycler instrument
- intended for testing in Caucasian population
- intended for laboratory professional use by trained healthcare professionals
- the proof of the presence of the HLA-B27 serves as an aid to diagnosis of patients with suspected pathological state caused by presence of B*27 allele associated with inflammatory rheumatologic conditions (axial spondyloarthritis also known as ankylosing spondylitis or Bechterew´s disease)
Clinical implications
The presence of B27 antigen (HLA-B27) is strongly associated with ankylosing spondylitis (AS) and other inflammatory disorders. The frequency of HLA-B*27 allele in Caucasian population is in range 5–10% and in AS-patients occurs in more than 85% cases. The absence of HLA-B27 allele is a strong negative predictor for diagnosis of AS.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3257-025 | gb GENETIC HLA-B*27 | 25 |
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| 3257-100 | gb GENETIC HLA-B*27 | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction)
Shiping on dry ice from
€ 115 (EU)
For other destinations see
indicative list.
Validated for:
CFX96/96Touch (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
The human leukocyte antigen HLA-B27 is a variant of the class I major histocompatibility complex (MHC I) molecule. It is encoded by the HLA-B locus on chromosome 6 and expressed on almost all nucleated cells. Role in Health and Disease HLA-B27’s normal function is to present peptide antigens (derived from both self and non-self proteins)
gb GENETIC HFE
The CE IVD real-time PCR kit intended for qualitative genotyping of the rs1800562 (C282Y), rs1799945 (H63D) and rs1800730 (S65C) polymorphisms in the human gene HFE encoding the hereditary hemochromatosis protein (HFE).
gb GENETIC APOE
The CE IVD real-time PCR kit intended for qualitative genotyping of the rs429358 (112R) and rs7412 (158C) polymorphisms in the human gene APOE encoding the apolipoprotein E.