Home Products gb GENETIC HLA-B*27

gb GENETIC HLA-B*27

Detection method: real-time PCR

The kit is intended for detection of HLA-B27 allele in samples of human DNA. Detection principle is based on real-time PCR method with allele specific primers and fluorescently labeled hydrolyzation probes.

Clinical implications

The presence of B27 antigen (HLA-B27) is strongly associated with ankylosing spondylitis (AS) and other inflammatory disorders. The frequency of HLA-B*27 allele in Caucasian population is in range 5–10% and in AS-patients occurs in more than 85% cases. The absence of HLA-B27 allele is a strong negative predictor for diagnosis of AS.

Parameters of the diagnostic kit

in vitro diagnostics
CE IVD marked
ready-to-use assay
sample concentration 1-100 ng/µl
positive and negative controls included
FAM and HEX channels detection
identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits

View allView less

Cat. No.	Product	No. of reactions	Price

3257-025	*gb GENETIC HLA-B27**	25	Add to Quote Add to Quote
Get this product FREE Get up to 2 different products as free samples, Pay only shipping.			Order for free

3257-100

gb GENETIC HLA-B*27

100

Add to Quote
Add to Quote

1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction)

Shiping on dry ice from € 115 (EU)
For other destinations see indicative list.

Download Product Information Download Full Catalogue

Search for documents by LOT number

General Terms and Conditions

Validated for:

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

QuantStudio 5 (Applied Biosystems)

RG 3000 (Corbett Research)

Select further cyclers

Useful links

gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.