Detection method: allelic discrimination
This in vitro diagnostic kit is intended for detection of mutations LCT (C13910T) and LCT (G22018A) in the lactase gene in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Genetically determined lactose intolerance is a disorder of the metabolism of lactose (milk sugar) caused by decreased production of the lactase enzyme. Under usual conditions small intestinal cells produce lactase, which decomposes lactose into simpler sugars – glucose and galactose. These are then absorbed by the wall of the small intestine, getting into the bloodstream, and serves as an energy source.
In case of lactose intolerance the undigested lactose is accumulated in the intestine and then decomposed by intestinal bacteria. Gastrointestinal symptoms include diarrhea, abdominal discomfort, stomach cramps or bloating as a result of lactose degradation. In Europe, lactose intolerance is associated with two mutations in the regulatory region of the gene LCT at positions -13910 and -22018. This gene is responsigb ble for the level of the enzyme lactase. In the presence of alleles LCT -13910C and LCT -22018G the activity of lactase gene is much lower, which leads to lactase non-persistence. Haplotype LCT -13910T and -22018A are conversely associated with lactase persistence.
| Cat. No. | Product | No. of reactions | Price |
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| 3219-025 | gb GENETIC LACTO | 25 |
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| 3219-050 | gb GENETIC LACTO | 50 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
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Validated for:
ABI 7300 (ABI)
ABI 7500/7500 Fast (ABI)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
SmartCycler (Cepheid)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
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Products are dispatched within 10 business days upon receiving the order
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Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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