Detection method: allelic discrimination
This in vitro diagnostic kit is intended for detection of mutations LCT (C13910T) and LCT (G22018A) in the lactase gene in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Genetically determined lactose intolerance is a disorder of the metabolism of lactose (milk sugar) caused by decreased production of the lactase enzyme. Under usual conditions small intestinal cells produce lactase, which decomposes lactose into simpler sugars – glucose and galactose. These are then absorbed by the wall of the small intestine, getting into the bloodstream, and serves as an energy source.
In case of lactose intolerance the undigested lactose is accumulated in the intestine and then decomposed by intestinal bacteria. Gastrointestinal symptoms include diarrhea, abdominal discomfort, stomach cramps or bloating as a result of lactose degradation. In Europe, lactose intolerance is associated with two mutations in the regulatory region of the gene LCT at positions -13910 and -22018. This gene is responsigb ble for the level of the enzyme lactase. In the presence of alleles LCT -13910C and LCT -22018G the activity of lactase gene is much lower, which leads to lactase non-persistence. Haplotype LCT -13910T and -22018A are conversely associated with lactase persistence.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3219-025 | gb GENETIC LACTO | 25 |
Add to Quote Add to Quote |
|
Get this product FREE |
Order for free | ||
| 3219-050 | gb GENETIC LACTO | 50 |
Add to Quote Add to Quote |
1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (20 μl volume of each reaction).
Shiping on dry ice from
€ 110 (EU)
For other destinations see
indicative list.
General Terms and Conditions
Validated for:
ABI 7300 (ABI)
ABI 7500/7500 Fast (ABI)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
SmartCycler (Cepheid)
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional
The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
Biodistribution (BD) studies are critical components when assessing the preclinical safety evaluation of cell and gene therapy molecules. BD analysis is generally conducted at the molecular level using a methodology that provides high sensitivity.
Your request has been sent.
Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
This product has not been validated for your cycler?
Send us the type of your cycler and we will find a solution for you.
Your request has been sent.
Thank you for your interest in our testing services. We will reply to your e-mail address as soon as possible.
Thank you for your inquiry
Thank you for your inquiry
Your registration has been sent.
Thank you for your inquiry
Price will be calculated based on your exact location within 2 working days.
ENG 
CZE 
