Detection method: quantitative real-time PCR
This kit is intended for detection of the specific section of the SRY gene which is located on the Y chromosome in the human genomic DNA. Detection is based on quantitative realtime polymerase chain reaction (qPCR) using fluorescently labelled probes.
The quantification of human DNA is significant especially in the field of forensic analysis. The SRY gene is located on the short arm of human chromosome Y in the position 11.3. This gene encodes a transcription factor that binds to specific region of protein. This protein called „sex-determining region Y“ is the testis-determining factor, which initiates male sex determination.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3143 | gb GENETIC SRY | 100 |
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1 RUO real-time PCR kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).
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€ 110 (EU)
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General Terms and Conditions
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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