gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.
gb GENETIC SRY
Detection method: quantitative real-time PCR
This kit is intended for detection of the specific section of the SRY gene which is located on the Y chromosome in the human genomic DNA. Detection is based on quantitative realtime polymerase chain reaction (qPCR) using fluorescently labelled probes.
Clinical implications of the RUO kit
The quantification of human DNA is significant especially in the field of forensic analysis. The SRY gene is located on the short arm of human chromosome Y in the position 11.3. This gene encodes a transcription factor that binds to specific region of protein. This protein called „sex-determining region Y“ is the testis-determining factor, which initiates male sex determination.
Parameters of the real-time PCR diagnostic kit
- ready-to-use assay
- positive and negative controls included
- detection in FAM channel
- internal positive control in HEX channel
|Cat. No.||Product||No. of reactions||Price|
|3143||gb GENETIC SRY||100||
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1 RUO real-time PCR kit contains reagents to provide 100 PCR reactions (25 μl volume of each reaction).
Shiping on dry ice from
€ 105 (EU)
For other destinations see indicative list.