Detection method: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of A4600G mutation in EPCR gene (haplotype H3) in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Endothelial protein C receptor (EPCR) is a transmembrane protein expressed in large vessel endothelial cells. Its traces can be found in most capillaries. It binds protein C on the surface of endothelial cells with high affinity, thus accelerating its activation. Protein C is activated by means of the thrombin-thrombomodulin complex and regulates thrombin production by inactivation of Factors Va and VIIIa.
Common production of activated protein C (APC) depends on the surface of endothelial cells, thrombin, thrombomodulin, protein C, and EPCR. Therefore, any change of the said components can increase or decrease APC production and influence the risk of thrombosis. EPCR A4600G polymorphism is the result of one-nucleotide A>G transition at nucleotide 4600 of the endothelial protein C receptor gene (haplotype H3). People with haplotype H3 have higher plasmatic concentration of EPCR, which is related to an increased risk of venous thrombosis.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3215-025 | gb HEMO EPCR (A4600G) | 25 |
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| 3215 | gb HEMO EPCR (A4600G) | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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indicative list.
Validated for:
ABI 7500/7500 Fast (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
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Dispatch date
Products are dispatched within 5 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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