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gb HEMO EPCR (A4600G)

Detection method: allelic discrimination, real-time PCR

This in vitro diagnostic kit is intended for detection of A4600G mutation in EPCR gene (haplotype H3) in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).

Clinical implications of the CE IVD kit

Endothelial protein C receptor (EPCR) is a transmembrane protein expressed in large vessel endothelial cells. Its traces can be found in most capillaries. It binds protein C on the surface of endothelial cells with high affinity, thus accelerating its activation. Protein C is activated by means of the thrombin-thrombomodulin complex and regulates thrombin production by inactivation of Factors Va and VIIIa.

Common production of activated protein C (APC) depends on the surface of endothelial cells, thrombin, thrombomodulin, protein C, and EPCR. Therefore, any change of the said components can increase or decrease APC production and influence the risk of thrombosis. EPCR A4600G polymorphism is the result of one-nucleotide A>G transition at nucleotide 4600 of the endothelial protein C receptor gene (haplotype H3). People with haplotype H3 have higher plasmatic concentration of EPCR, which is related to an increased risk of venous thrombosis.

Parameters of the real-time PCR diagnostic kit

ready-to-use assay
sample concentration 1-100 ng/µl
positive and negative controls included
FAM and HEX channels detection
identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits

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Cat. No.	Product	No. of reactions	Price

3215-025

gb HEMO EPCR (A4600G)

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3215

gb HEMO EPCR (A4600G)

100

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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 95 (EU)
For other destinations see indicative list.

Terms & Conditions Delivery Conditions

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Validated for:

ABI 7500/7500 Fast (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

MIC (BMS)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

Select further cyclers

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