Categories

gb HEMO FXIII (V34L) gb HEMO FXIII (V34L)

gb HEMO FXIII (V34L)

Detection method: allelic discrimination, real-time PCR

This in vitro diagnostic kit is intended for detection of mutation V34L in fibrin stabilizing factor XIII in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).

Clinical implications of the CE IVD kit

Factor XIII, also called fibrin stabilizing factor, is a proenzym circulating in plasma. Its active form stabilizes the fibrin network by catalytic formation of covalent bonds between fibrin molecules and other proteins (alpha-2-antiplasmin, fibronectin, collagen), thereby protecting the coagulum against fibrinolysis.

FXIIIa plays an important role not only in hemostasis but also during wound healing and in maintaining pregnancy. The presence of the V34L has a protective effect against myocardial infarction and venous thrombosis. On the other hand, it is also stated that the presence of this polymorphism is associated with an increased risk of recurrent pregnancy loss in the first trimester, which was also found in connection with the PAI-1 (4G/5G) mutation.

Parameters of the real-time PCR diagnostic kit

  • ready-to-use assay
  • sample concentration 1-100 ng/µl
  • positive and negative controls included
  • FAM and HEX channels detection
  • identical amplification profile as gb HEMO, gb GENETIC, gb PHARM kits
Cat. No. Product No. of reactions Price
3209-025 gb HEMO FXIII (V34L) 25
Add to Quote
Add to Quote
3209 gb HEMO FXIII (V34L) 100
Add to Quote
Add to Quote

1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 95 (EU)
For other destinations see indicative list.

Validated for:

ABI 7500/7500 Fast (ABI)

ABI 7900HT (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

MIC (BMS)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

SmartCycler (Cepheid)

Select further cyclers

Useful links
Recommended products
Kit for isolation of DNA from tissues

Kit for isolation of DNA from tissues Kit for isolation of DNA from tissues

The DNA extraction kit includes DNA purification step and enables achieving highly pure genomic DNA from various types of human or animal tissues and cultured cells, buccal swabs or paraffin-embedded tissue. Moreover, microbial, fungal or yeast DNA can be efficiently isolated as well. High DNA yields, reproducible results and fast working method are guaranteed.

More
Recommended articles
CRO case studies

Yeast Production Strain Cell Line Authentication by RFLP analysis

Yeast Production Strain Cell Line Authentication by RFLP analysis

Customer need Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of

More