Detection method: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of mutation V34L in fibrin stabilizing factor XIII in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Factor XIII, also called fibrin stabilizing factor, is a proenzym circulating in plasma. Its active form stabilizes the fibrin network by catalytic formation of covalent bonds between fibrin molecules and other proteins (alpha-2-antiplasmin, fibronectin, collagen), thereby protecting the coagulum against fibrinolysis.
FXIIIa plays an important role not only in hemostasis but also during wound healing and in maintaining pregnancy. The presence of the V34L has a protective effect against myocardial infarction and venous thrombosis. On the other hand, it is also stated that the presence of this polymorphism is associated with an increased risk of recurrent pregnancy loss in the first trimester, which was also found in connection with the PAI-1 (4G/5G) mutation.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3209-025 | gb HEMO FXIII (V34L) | 25 |
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| 3209 | gb HEMO FXIII (V34L) | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
ABI 7500/7500 Fast (ABI)
ABI 7900HT (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
SmartCycler (Cepheid)
gb Easy PCR Master Mix gb Easy PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. The Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR. The product falls into a group of TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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