Detection method: allelic discrimination, real-time PCR
This in vitro diagnostic kit is intended for detection of L33P mutation in GPIIIa (ITGB3) gene in human genomic DNA. Detection is based on real-time polymerase chain reaction (qPCR) using fluorescently labelled probes (allelic discrimination).
Platelet glycoprotein GPIIIa can be found on the surface of blood platelets in form of a complex GPIIb/IIIa. It belongs to the family of adhesive receptors. The interaction of the said complex with fibrinogen plays a key role in thrombocyte adhesion to the surfaces and each other. L33P polymorphism is the most common and clinically most significant polymorphism of the said gene.
One-nucleotide T>C transition leads to a substitution of the amino acid leucine for proline at nucleotide 33. Mutated allele called PLA2 leads to an increase of thrombocyte aggregability. The carriage of the mutated allele is considered to be a mild risk factor of the coronary artery disease and myocardial infarction in younger people and pre-eclampsia in pregnant women. The
level of risk is increased at concurrent occurrence of other polymorphisms (eNOS, PAI-1). The homozygous carriers of the mutated allele show more frequently reduced susceptibility to antiplatelet therapy by clopidogrel or acetylsalicylic acid (aspirin resistance).
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3212-025 | gb HEMO GPIIIa (L33P) | 25 |
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| 3212 | gb HEMO GPIIIa (L33P) | 100 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 100 PCR reactions (20 μl volume of each reaction).
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General Terms and Conditions
Validated for:
ABI 7300 (ABI)
ABI 7500/7500 Fast (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
Light Cycler 480/Cobas z480 (Roche Diagnostics)
MIC (BMS)
QuantStudio 5 (Applied Biosystems)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
Dual-labeled hydrolysis probes The technology of dual-labeled hydrolysis probes (also called TaqMan® real-time PCR probes) exploits the 5 ́-3 ́ exonuclease activity of Taq DNA polymerase to cleave the probe. TaqMan real-time PCR probes are labeled with a fluorescent dye (reporter) at the 5´ end and with a quencher at the 3´ end.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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