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gb Ideal PCR Master Mix gb Ideal PCR Master Mix

gb Ideal PCR Master Mix

gb Ideal PCR Master Mix is intended for research PCR and real-time PCR applications with fluorescently labelled probes and with high sensitivity and efficiency request. It is not intended for use in diagnostics.

Description of the product

gb Ideal PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP and MgCl2. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Parameters of the product

  • Master Mix is a 2× concentrated solution.
  • It contains all the components necessary for PCR performance.
  • It is specified especially for real-time PCR applications demanding high sensitivity, for example microbial DNA detection and somatic mutations detection. Master Mix is enriched with components which preventPCR reaction inhibition through which higher reliability of the detection is ensured.
  • Polymerase is a hot-start type with a short activation time (3 min / 95 °C), with 5´-3´ polymerase and exonuclease activity, 3´-5´ exonuclease activity is not present.

 

gb PCR Master Mix by application
 gb Ideal
end-point PCR, common PCR amplification
real-time PCR without probes
real-time PCR with hydrolysis probes

✓
real-time PCR with LNA probes

✓
real-time PCR with hybridization probes
real-time PCR with High Resolution Melting Analysis
real-time PCR with low DNA samples

✓
real-time PCR with inhibited samples
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Cat. No. Product No. of reactions Price
3007 gb Ideal PCR Master Mix 100
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1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).

Shiping on dry ice from € 115 (EU)
For other destinations see indicative list.

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General Terms and Conditions

Useful links
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Passive reference dye is an inert dye which serves as inner reference dye for fluorescence signal normalization between the wells which can occur as a result of errors in pipetting or under the influence of device limitations. It is used with TaqMan Real-Time PCR Master Mixes to provide probe-based qPCR or with probe-based One-Step qRT-PCR.

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Delivery terms – Master Mixes

Dispatch date
Products are dispatched within 10 business days upon receiving the order

Shipping
On ice/dry ice

Recommended storage
Storage at -20 °C

Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)

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Oligos with this modification can be inquired or directly ordered using oligo portal.

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Probes of this type can be inquired or directly ordered using oligo portal.

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Oligos with this modification can be ordered only after previous offer. After specific sequences are input to oligo portal (log-in here) we will make an exact offer that you can simply use for your order.

If you have not been registered in oligo portal, you can make registration here.

Probes of this type can be ordered only after previous offer. After specific sequences are input to oligo portal (log-in here) we will make an exact offer that you can simply use for your order.

If you have not been registered in oligo portal, you can make registration here.

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