gb IPC PCR Master Mix is intended for real-time PCR applications with fluorescently labelled probes and with high sensitivity and efficiency request. Its advantages are the presence of internal positive control used to check the correctness of PCR, and also increased resistance towards PCR inhibitors. It is not intended for use in diagnostics.
gb IPC PCR Master Mix consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2 and additives that prevent PCR inhibition and Internal positive control (IPC). IPC included in the Master Mix serves to verify that the PCR procedure was done properly. With the use of IPC it is possible to distinguish false negative DNA samples (negativity is caused by improper performance of PCR reaction or inhibition of PCR reaction) from real negative sample. The fluorescence signal of the IPC is detected in HEX/JOE/VIC channel.
| gb PCR Master Mix by application |
gb IPC |
| end-point PCR, common PCR amplification | |
| real-time PCR without probes | |
| real-time PCR with hydrolysis probes |
✓ |
| real-time PCR with LNA probes | |
| real-time PCR with hybridization probes | |
| real-time PCR with High Resolution Melting Analysis | |
| real-time PCR with low DNA samples |
✓ |
| real-time PCR with inhibited samples |
✓ |
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3013 | gb IPC PCR Master Mix | 100 |
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1 tube contains reagents to provide 100 PCR reactions (20 μl volume of each reaction).
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€ 110 (EU)
For other destinations see
indicative list.
General Terms and Conditions
PCR users have sometimes trouble with their real-time PCR experiments. Their real-time PCR curves give no signal or they have complication with signal in negative control. We will try to help users with the main reasons of your PCR troubles and how to solve them.
gb Elite Reverse Transcription Kit gb Elite Reverse Transcription Kit is intended for reverse transcription using enzyme MMLV reverse transcriptase. This enzyme converts RNA to cDNA (complementary DNA) in one step (without denaturation), you only have to add your template RNA.
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Dispatch date
Products are dispatched within 10 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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