Detection method: quantitative real-time PCR
This in vitro diagnostic kit is intended for detection and DNA quantification of Herpes simplex virus 2 (HSV2) by real-time PCR method.
Herpes simplex virus 2 (HSV2) is an enveloped DNA-virus of Herpesviridae family belonging to the Simplexvirus genus. For these viruses a short replication time and the ability to
trigger latent infection of cells is typical. HSV2 virus enters the human body through the mucosis of the genitals. The virus proliferates in the entrance gate and extends further on to the nerve endings.
Then it arrives in the ganglia cells by axonal transport where it lasts for practically the whole life. Reactivation of infection and repetitive proliferation of the virus, so called recurrent infection, can happen under the influence of different types of stress (fever, hormonal influences, insolation etc). Primary infection triggered by HSV2 virus is demonstrated in adults as herpes genitalis, it can occur as generalized herpes with afflictions of the skin, brain, liver and lungs of newly born babies of non immune mothers. The course of recurrent infection of HSV2 is usually much milder than at the primary infection.
| Cat. No. | Product | No. of reactions | Price |
|---|
| 3224-025 | gb MICRO Herpes simplex virus 2 | 25 |
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| 3224-050 | gb MICRO Herpes simplex virus 2 | 50 |
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (25 μl volume of each reaction).
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Validated for:
ABI 7500/7500 Fast (ABI)
ABI 7900HT (ABI)
AriaMx (Agilent Technologies)
CFX96/96Touch (Bio-Rad)
iCycler iQ5 (Bio-Rad)
RG 3000 (Corbett Research)
RG 6000/Q (Corbett Research/Qiagen)
gb OneStep RT-qPCR Kit gb OneStep RT-qPCR Kit falls into a group of TaqMan Real-Time PCR Master Mixes. It provides cDNA synthesis in one-step. The product is intended for reverse transcription (RT) and real-time PCR (qPCR) performed in one step (in one reaction), i.e. onestep RT-qPCR. No additional instrumentation is required.
Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.
Background Probiotics are defined as live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. For instance, probiotics can be used in the treatment and prevention of infections and chronic inflammatory disorders of the gastrointestinal tract. Due to their health attributes, probiotics generate considerable interest in the area of functional
The avermectins compose a class of polycyclic lactones that are used to treat parasitic worms and insect pests. They are naturally occurring compounds generated in a fermentation process by a soil actinomycete Streptomyces avermitilis.
Biodistribution (BD) studies are critical components when assessing the preclinical safety evaluation of cell and gene therapy molecules. BD analysis is generally conducted at the molecular level using a methodology that provides high sensitivity.
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Dispatch date
Products are dispatched within 5 business days upon receiving the order
Shipping
On ice/dry ice
Recommended storage
Storage at -20 °C
Expiry date
Product remains stable until expiry date stated on the product label (min. 6 months after delivery)
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