gb MICRO Herpes simplex virus 2 gb MICRO Herpes simplex virus 2

gb MICRO Herpes simplex virus 2

Detection method: quantitative real-time PCR

This in vitro diagnostic kit is intended for detection and DNA quantification of Herpes simplex virus 2 (HSV2) by real-time PCR method.

Clinical implications of the CE IVD kit

Herpes simplex virus 2 (HSV2) is an enveloped DNA-virus of Herpesviridae family belonging to the Simplexvirus genus. For these viruses a short replication time and the ability to
trigger latent infection of cells is typical. HSV2 virus enters the human body through the mucosis of the genitals. The virus proliferates in the entrance gate and extends further on to the nerve endings.

Then it arrives in the ganglia cells by axonal transport where it lasts for practically the whole life. Reactivation of infection and repetitive proliferation of the virus, so called recurrent infection, can happen under the influence of different types of stress (fever, hormonal influences, insolation etc). Primary infection triggered by HSV2 virus is demonstrated in adults as herpes genitalis, it can occur as generalized herpes with afflictions of the skin, brain, liver and lungs of newly born babies of non immune mothers. The course of recurrent infection of HSV2 is usually much milder than at the primary infection.

Parameters of the real-time PCR diagnostic kit

  • ready-to-use assays
  • LOD 0.57 copies/µl
  • internal positive control, standards and negative control included
  • FAM channel detection
  • internal positive control in HEX channel
Cat. No. Product No. of reactions Price
3224-025 gb MICRO Herpes simplex virus 2 25
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3224-050 gb MICRO Herpes simplex virus 2 50
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1 CE IVD real-time PCR kit contains reagents to provide 25 or 50 PCR reactions (25 μl volume of each reaction).

Shiping on dry ice from € 100 (EU)
For other destinations see indicative list.

Validated for:

ABI 7500/7500 Fast (ABI)

ABI 7900HT (ABI)

AriaMx (Agilent Technologies)

CFX96/96Touch (Bio-Rad)

iCycler iQ5 (Bio-Rad)

RG 3000 (Corbett Research)

RG 6000/Q (Corbett Research/Qiagen)

Select further cyclers

Useful links
Recommended products
gb SG PCR Master Mix

gb SG PCR Master Mix gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

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