Home Products gb ONCO BCR-ABL MICRO / ABL

gb ONCO BCR-ABL MICRO / ABL

Detection method: one-step real-time PCR

This in vitro diagnostic kit enables the quantitative determination of the copy number ratio of the e19a2 (c3a2) transcript of the BCR-ABL1 fusion gene to the copy number of the ABL1 reference gene transcript.

Clinical implications of the CE IVD kit

The BCR-ABL1 fusion gene in the rare MICRO variant occurs in patients with chronic neutrophilic leukemia (CML-N). Patient response to treatment as well as minimal residual disease is monitored by quantification of BCR-ABL1 transcript relative to control gene transcript (ABL1).

Parameters of the real-time PCR diagnostic kit

detection in the FAM (BCR-ABL1) channel and HEX (ABL1) channel
calibrating panel of standards and no template control included

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Cat. No.	Product	No. of reactions	Price

3248-048

gb ONCO BCR-ABL MICRO / ABL

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1 CE IVD one-step real-time PCR kit contains reagents to provide 48 PCR reactions (25 μl volume of each reaction).

Shiping on dry ice from € 110 (EU)
For other destinations see indicative list.

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About product

General Terms and Conditions

Validated for:

ABI 7500/7500 Fast (ABI)

CFX96/96Touch (Bio-Rad)

Light Cycler 480/Cobas z480 (Roche Diagnostics)

QuantStudio 5 (Applied Biosystems)

QuantStudio 7 (Applied Biosystems)

RG 3000 (Corbett Research)

Select further cyclers

Useful links

gb SG PCR Master Mix

gb SG PCR Master Mix falls into a group of Real-Time PCR Master Mixes to provide Dye-based qPCR. The product consists of hot-start Taq DNA polymerase, reaction buffer, dNTP, MgCl2, sybrgreen and additives that prevent PCR inhibition. Taq polymerase is chemically modified DNA polymerase from Thermus aquaticus. This polymerase is completely inactive at room temperature but it is rapidly activated during the initial denaturation step of PCR.

Alternative dyes for oligonucleotides/probes labeling

Why choose alternative dyes instead of "genuine" dyes? First, they are cheaper, and second, they can give the same results. Another reason is that the number of suppliers who can provide modified oligonucleotides is restricted - only a patent or a licence owner is usually allowed to produce them.